Healing of diabetic wounds present a big challenge due to insufficient vascular supply and bacterial infection. We developed chitosan based biodegradable polymeric hydrogel containing silver nanoparticles (AgNPs) as antimicrobial agent and calcium alginate nanoparticles (Ca-AlgNps) as hemostatic agent to address this problem. The prepared Chitosan/Ca-AlgNps/AgNPs hydrogel showed broad spectrum antimicrobial properties against both Gram negative (E. coli, P. aeruginosa) and Gram positive (B. subtilis, S. aureus) bacteria. Taken into account the blood as a vital material containing various circulatory fibrocytes, growth factors, cytokines, platelets and macrophages etc., we incorporated the fresh blood of the same animal to the prepared Chitosan/Ca-Alg Nps/AgNPs hydrogel. In-vivo animal studies of Chitosan/Ca-AlgNps/AgNPs hydrogel and blood mixed Chitosan/Ca-AlgNps/AgNPs hydrogel exhibit 83.5 ± 4.4% and 99.8 ± 2.0% closure of wound respectively, on day 15 as compared to 41.5 ± 3.2% in diabetic control and 60.3 ± 2.2% in commercially available wound healing cream, Silverex Heal. The incorporation of fresh blood to the prepared hydrogel has advantage in terms of supplying growth factors, platelets, circulatory fibrocytes and cytokines which further enhanced the wound healing mechanism in diabetic rats. This work opens a novel idea to formulate hydrogels based dressings for diabetic wound healing.

由于血管供应不足和细菌感染，糖尿病伤口的愈合是一个很大的挑战。我们开发了基于壳聚糖的可生物降解聚合物水凝胶，其中含有银纳米颗粒 (AgNPs) 作为抗菌剂和海藻酸钙纳米颗粒 (Ca-AlgNps) 作为止血剂来解决这个问题。制备的壳聚糖/Ca-AlgNps/AgNPs 水凝胶对革兰氏阴性菌（大肠杆菌、铜绿假单胞菌）和革兰氏阳性菌（枯草芽孢杆菌、金黄色葡萄球菌）均显示出广谱抗菌特性。考虑到血液作为重要物质，含有各种循环纤维细胞、生长因子、细胞因子、血小板和巨噬细胞等。，我们将同一动物的新鲜血液加入到制备的壳聚糖/Ca-Alg Nps/AgNPs 水凝胶中。壳聚糖/Ca-AlgNps/AgNPs 水凝胶和血液混合壳聚糖/Ca-AlgNps/AgNPs 水凝胶的体内动物研究在第 15 天分别表现出 83.5 ± 4.4% 和 99.8 ± 2.0% 的伤口闭合，与 41.5 ± 3.2% 相比在糖尿病控制和 60.3 ± 2.2% 的市售伤口愈合霜 Silverex Heal 中。在制备的水凝胶中加入新鲜血液在提供生长因子、血小板、循环纤维细胞和细胞因子方面具有优势，进一步增强了糖尿病大鼠的伤口愈合机制。这项工作开辟了一个新的想法来配制基于水凝胶的敷料用于糖尿病伤口愈合。