Using half-leaf method O,O′-diisopropyl (3-(L-1-(benzylamino)-1-oxo-3- phenylpropan-2-yl)thioureido)(phenyl)methyl phosphonate (2009104) was studied for its activity on tobacco mosaic virus (TMV). It showed good curative activity in vivo and the curative activity at 500 μg/mL was found to be 53.3%. In vivo treatment with the control agent Ningnanmycin at 500 μg/mL resulted in 51.2% inhibition and curative inhibition rates respectively. Dot-ELISA test was employed to verify the efficacy of activity of compound 200910 for anti-TMV activity. The mechanism of action of compound 2009104 to resist TMV was also studied. The results showed that the resistance enzymes PAL, POD, SOD activity and chlorophyll content after TMV inoculation K326 (Nicotiana tabacum K326) of tobacco plants followed by treatment with compound 2009104 were significantly enhanced. The study of the effect of compound 2009104 on TMV capsid protein (CP) showed that it inhibited the polymerization process of TMV-CP in vitro.

中文翻译：

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新型手性膦酸酯硫脲对烟草花叶病毒的抗病毒活性及作用机理

用半叶法研究了O，O'-二异丙基（3-（L-1-（苄氨基）-1-氧代-3-苯基丙烷-2-基）硫脲基）（苯基）甲基膦酸酯（2009104）的活性在烟草花叶病毒（TMV）上。它显示出良好的体内治疗活性，发现在500μg/ mL下的治疗活性为53.3％。对照剂宁南霉素在500μg/ mL的体内处理分别产生51.2％的抑制率和治愈率。采用Dot-ELISA测试来验证化合物200910的活性对于抗TMV活性的功效。还研究了化合物2009104抵抗TMV的作用机理。结果表明，TMV接种烟草植株K326（烟草K326）后再用化合物2009104处理后，抗性酶PAL，POD，SOD活性和叶绿素含量显着提高。