MiR-99b-5p 通过靶向 3T3-L1 细胞中的 SCD1 和 Lpin1 减弱脂肪生成,Journal of Agricultural and Food Chemistry

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MiR-99b-5p 通过靶向 3T3-L1 细胞中的 SCD1 和 Lpin1 减弱脂肪生成
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-02-18 , DOI: 10.1021/acs.jafc.0c07451
Yanting Xu ₁ , Xiaochang Chen ₁ , Chen Zhao ₁ , Xiaoting Wang ₁ , Ye Cheng ₁ , Fengxue Xi ₁ , Xiangping Yao ₁ , Lei Zhang ₁ , Gongshe Yang ₁ , Taiyong Yu ₁

Affiliation

脂肪细胞的数量和分布直接影响畜肉产品的品质。脂肪生成机制的分析是改善肉质的基础。脂肪细胞的形成受许多因素的调节，包括一类称为 microRNA (miRNA) 的内源性小 RNA。先前的研究表明，miRNA 可以通过靶基因的转录后调控来影响脂肪生成。在我们的研究中，在肥胖小鼠的脂肪组织中发现 miR-99b-5p 表达水平降低。miR-99b-5p的过表达可以通过促进细胞周期同时抑制细胞分化来增加细胞增殖。此外，干扰 miR-99b-5p 得到了相反的结果。此外，蛋白质组学测序分析筛选了 1154 种差异表达的蛋白质，与脂肪细胞分化和脂肪酸代谢密切相关。此外，双荧光素酶检测结果表明，在蛋白质组测序中，miR-99b-5p可以直接靶向表达水平差异显着的蛋白质SCD1和Lpin1。然后，在进一步的研究中，这一结果在 mRNA 和蛋白质水平上得到了验证。总的来说，这些结果表明 miR-99b-5p 可能是改善肉质的目标。

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MiR-99b-5p Attenuates Adipogenesis by Targeting SCD1 and Lpin1 in 3T3-L1 Cells

The number and distribution of adipocytes directly affect the quality of livestock meat products. The analysis of the adipogenesis mechanism is the basis for improving meat quality. The formation of adipocytes is regulated by many factors, including a class of endogenous small RNAs, named microRNA (miRNA). Previous studies have shown that miRNAs could affect adipogenesis by post-transcriptional regulation of target genes. In our study, a decreased miR-99b-5p expression level was found in the adipose tissue of obese mice. Overexpression of miR-99b-5p could increase cell proliferation by promoting the cell cycle while inhibiting cell differentiation. In addition, interference with miR-99b-5p obtained the opposite result. Furthermore, the proteomics sequencing analysis screened 1154 differentially expressed proteins, which are closely related to adipocyte differentiation and fatty acid metabolism. In addition, the results of the dual-luciferase test showed that miR-99b-5p can directly target the proteins SCD1 and Lpin1 with significantly different expression levels in proteomic sequencing. Then, this result was verified at the level of mRNA and protein in a further study. Collectively, these results suggested that miR-99b-5p may be a target for improving meat quality.

更新日期：2021-03-03

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