Vibrational Spectroscopic Detection of a Single Virus by Mid-Infrared Photothermal Microscopy,Analytical Chemistry

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Vibrational Spectroscopic Detection of a Single Virus by Mid-Infrared Photothermal Microscopy
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-02-17 , DOI: 10.1021/acs.analchem.0c05333
Yi Zhang ₁ , Celalettin Yurdakul ₂ , Alexander J Devaux ₃ , Le Wang _{2,

4} , Xiaoji G Xu ₄ , John H Connor ₃ , M Selim Ünlü _{1,

2,

5} , Ji-Xin Cheng _{1,

2,

5}

Affiliation

We report a confocal interferometric mid-infrared photothermal (MIP) microscope for ultra-sensitive and spatially resolved chemical imaging of individual viruses. The interferometric scattering principle is applied to detect the very weak photothermal signal induced by infrared absorption of chemical bonds. Spectroscopic MIP detection of single vesicular stomatitis viruses (VSVs) and poxviruses is demonstrated. The single virus spectra show high consistency within the same virus type. The dominant spectral peaks are contributed by the amide I and amide II vibrations attributed to the viral proteins. The ratio of these two peaks is significantly different between VSVs and poxviruses, highlighting the potential of using interferometric MIP microscopy for label-free differentiation of viral particles. This all-optical chemical imaging method opens a new way for spectroscopic detection of biological nanoparticles in a label-free manner and may facilitate in predicting and controlling the outbreaks of emerging virus strains.

中文翻译：

中红外光热显微镜对单一病毒的振动光谱检测

我们报告了一种用于对单个病毒进行超灵敏和空间分辨化学成像的共焦干涉中红外光热 (MIP) 显微镜。干涉散射原理用于检测化学键红外吸收引起的非常微弱的光热信号。证明了单个水泡性口炎病毒 (VSV) 和痘病毒的光谱 MIP 检测。单一病毒谱在同一病毒类型内表现出高度一致性。主要光谱峰由归因于病毒蛋白的酰胺 I 和酰胺 II 振动贡献。这两个峰的比率在 VSV 和痘病毒之间存在显着差异，突出了使用干涉 MIP 显微镜对病毒颗粒进行无标记分化的潜力。

更新日期：2021-03-02

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