当前位置: X-MOL 学术ACS Appl. Mater. Interfaces › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Biofunction of Polydopamine Coating in Stem Cell Culture
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2021-02-17 , DOI: 10.1021/acsami.0c22565
Zijun Deng 1, 2 , Weiwei Wang 1 , Xun Xu 1 , Yan Nie 1, 3 , Yue Liu 1, 3 , Oliver E C Gould 1 , Nan Ma 1, 2 , Andreas Lendlein 1, 2, 3
Affiliation  

High levels of reactive oxygen species (ROS) during stem cell expansion often lead to replicative senescence. Here, a polydopamine (PDA)-coated substrate was used to scavenge extracellular ROS for mesenchymal stem cell (MSC) expansion. The PDA-coated substrate could reduce the oxidative stress and mitochondrial damage in replicative senescent MSCs. The expression of senescence-associated β-galactosidase of MSCs from three human donors (both bone marrow- and adipose tissue-derived) was suppressed on PDA. The MSCs on the PDA-coated substrate showed a lower level of interleukin 6 (IL-6), one of the senescence-associated inflammatory components. Cellular senescence-specific genes, such as p53 and p21, were downregulated on the PDA-coated substrate, while the stemness-related gene, OCT4, was upregulated. The PDA-coated substrate strongly promoted the proliferation rate of MSCs, while the stem cell character and differentiation potential were retained. Large-scale expansion of stem cells would greatly benefit from the PDA-coated substrate.

中文翻译:

聚多巴胺涂层在干细胞培养中的生物功能

干细胞扩增过程中高水平的活性氧(ROS)通常会导致复制性衰老。在这里,使用聚多巴胺(PDA)涂覆的底物清除细胞外ROS,促进间充质干细胞(MSC)扩增。覆有PDA的基质可以减少复制性衰老MSC中的氧化应激和线粒体损伤。在PDA上抑制了来自三个人类供体(骨髓和脂肪组织均衍生)的MSC的衰老相关β-半乳糖苷酶的表达。涂有PDA的基质上的MSC显示出较低水平的白细胞介素6(IL-6),这是与衰老相关的炎症成分之一。细胞衰老特异性基因,例如p53和p21,在PDA包被的底物上被下调,而干性相关基因OCT4被上调。涂有PDA的基质强烈地促进了MSC的增殖速率,同时保留了干细胞的特性和分化潜能。干细胞的大规模扩增将极大地受益于涂有PDA的基质。
更新日期:2021-03-10
down
wechat
bug