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Transcriptional changes revealed genes and pathways involved in the deficient testis caused by the inhibition of alkaline ceramidase (Dacer) in Drosophila melanogaster
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-02-15 , DOI: 10.1002/arch.21765
Min‐Jing Zhang 1 , Xiao‐Xiao Shi 1 , Ni Wang 1 , Chao Zhang 1 , Chunhong Zhang 1 , Md Khairul Quais 1 , Soomro Abid Ali 1 , Wenwu Zhou 1 , Cungui Mao 2 , Zeng‐Rong Zhu 1
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-02-15 , DOI: 10.1002/arch.21765
Min‐Jing Zhang 1 , Xiao‐Xiao Shi 1 , Ni Wang 1 , Chao Zhang 1 , Chunhong Zhang 1 , Md Khairul Quais 1 , Soomro Abid Ali 1 , Wenwu Zhou 1 , Cungui Mao 2 , Zeng‐Rong Zhu 1
Affiliation
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Sphingolipids are ubiquitous structural components of eukaryotic cell membranes which are vital for maintaining the integrity of cells. Alkaline ceramidase is a key enzyme in sphingolipid biosynthesis pathway; however, little is known about the role of the enzyme in the male reproductive system of Drosophila melanogaster. To investigate the impact of alkaline ceramidase (Dacer) on male Drosophila, we got Dacer deficiency mutants (MUs) and found they displayed apparent defects in the testis's phenotype. To profile the molecular changes associated with this abnormal phenotype, we performed de novo transcriptome analyses of the MU and wildtype (WT) testes; and revealed 1239 upregulated genes and 1102 downregulated genes. Then, six upregulated DEGs (papilin [Ppn], croquemort [Crq], terribly reduced optic lobes [Trol], Laminin, Wunen‐2, collagen type IV alpha 1 [Cg25C]) and three downregulated DEGs (mucin related 18B [Mur18B], rhomboid‐7 [Rho‐7], CG3168) were confirmed through quantitative real‐time polymerase chain reaction in WT and MU samples. The differentially expressed genes were mainly associated with catalytic activity, oxidoreductase activity and transmembrane transporter activity, which significantly contributed to extracellular matrix‐receptor interaction, fatty acids biosynthesis as well as glycine, serine, and threonine metabolism. The results highlight the importance of Dacer in the reproductive system of D. melanogaster and provide valuable resources to dig out the specific biological functions of Dacer in insect reproduction.
中文翻译：
转录变化揭示了果蝇黑alkaline中碱性神经酰胺酶（Dacer）的抑制所引起的睾丸缺陷相关基因和途径。
鞘脂是真核细胞膜的普遍存在的结构成分，对于维持细胞的完整性至关重要。碱性神经酰胺酶是鞘脂生物合成途径中的关键酶。然而，人们对这种酶在果蝇果蝇雄性生殖系统中的作用知之甚少。为了研究碱性神经酰胺酶（Dacer）对雄性果蝇的影响，我们得到了Dacer缺乏突变体（MUs），发现它们在睾丸表型中表现出明显的缺陷。为了剖析与此异常表型相关的分子变化，我们对MU和野生型（WT）睾丸进行了从头转录组分析。揭示了1239个上调的基因和1102个下调的基因。然后，六个上调的DEGs（木瓜蛋白酶[Ppn]，croquemort [Crq]，视神经叶明显减少[Trol]，层粘连蛋白，Wunen-2，IV型胶原蛋白α1 [Cg25C]）和三个下调的DEGs（与粘蛋白相关的18B [Mur18B] ，菱形7 [Rho-7]，CG3168）通过WT和MU样品中的实时定量聚合酶链反应进行了确认。差异表达的基因主要与催化活性，氧化还原酶活性和跨膜转运蛋白活性有关，这显着促进了细胞外基质-受体相互作用，脂肪酸的生物合成以及甘氨酸，丝氨酸和苏氨酸的代谢。结果突出了Dacer在D. melanogaster生殖系统中的重要性，并提供了宝贵的资源来挖掘Dacer在昆虫繁殖中的特定生物学功能。
更新日期：2021-03-11
中文翻译：
转录变化揭示了果蝇黑alkaline中碱性神经酰胺酶（Dacer）的抑制所引起的睾丸缺陷相关基因和途径。
鞘脂是真核细胞膜的普遍存在的结构成分，对于维持细胞的完整性至关重要。碱性神经酰胺酶是鞘脂生物合成途径中的关键酶。然而，人们对这种酶在果蝇果蝇雄性生殖系统中的作用知之甚少。为了研究碱性神经酰胺酶（Dacer）对雄性果蝇的影响，我们得到了Dacer缺乏突变体（MUs），发现它们在睾丸表型中表现出明显的缺陷。为了剖析与此异常表型相关的分子变化，我们对MU和野生型（WT）睾丸进行了从头转录组分析。揭示了1239个上调的基因和1102个下调的基因。然后，六个上调的DEGs（木瓜蛋白酶[Ppn]，croquemort [Crq]，视神经叶明显减少[Trol]，层粘连蛋白，Wunen-2，IV型胶原蛋白α1 [Cg25C]）和三个下调的DEGs（与粘蛋白相关的18B [Mur18B] ，菱形7 [Rho-7]，CG3168）通过WT和MU样品中的实时定量聚合酶链反应进行了确认。差异表达的基因主要与催化活性，氧化还原酶活性和跨膜转运蛋白活性有关，这显着促进了细胞外基质-受体相互作用，脂肪酸的生物合成以及甘氨酸，丝氨酸和苏氨酸的代谢。结果突出了Dacer在D. melanogaster生殖系统中的重要性，并提供了宝贵的资源来挖掘Dacer在昆虫繁殖中的特定生物学功能。
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