Introducing magnetic nanoparticles (MNP) into the enzyme@MOF complex is one of the most promising ways to facilitate enzyme recovery but typically hampered by its complex synthetic process and low loading rate. Using lipase as an example, herein, we reported a mild and quick one-pot strategy to achieve the successful encapsulation of lipase (CRL) and Fe₃O₄ nanoparticle into ZIF-8 to form CRL/MNP@ZIF-8 with 88.4 % immobilization yield. It is the first time that magnetic nanoparticles and lipase were simultaneously encapsulated in ZIF‐8 through the co‐precipitation approach. The resulting magnetic MOF composite demonstrated an excellent protective capability against harsh environments and could also simplify the complex downstream separation and recovery operations. Moreover, after five successive cycles of reuse to hydrolyze p-nitrophenyl acetate, the residual CRL/MNP@ZIF-8 still reserve 82 % activity, which can significantly reduce the processing cost and might be applicable in the industries in the future.

将磁性纳米颗粒（MNP）引入酶@MOF配合物中是促进酶回收的最有前途的方法之一，但通常会因其复杂的合成过程和低负载率而受阻。以脂肪酶为例，在本文中，我们报道了一种温和而快速的一锅法，以成功封装脂肪酶（CRL）和Fe ₃ O ₄纳米粒子进入ZIF-8形成CRL / MNP @ ZIF-8，固定化率为88.4％。这是首次通过共沉淀方法将磁性纳米颗粒和脂肪酶同时包裹在ZIF-8中。所得的磁性MOF复合材料在恶劣环境下表现出出色的防护能力，还可以简化复杂的下游分离和回收操作。此外，在连续五个循环重复使用以水解对硝基苯乙酸酯之后，残留的CRL / MNP @ ZIF-8仍保留82％的活性，这可以显着降低加工成本，并可能在未来的工业中应用。