发现Frenolicin B作为控制小麦<i>镰刀菌</i>病的潜在农用化学杀菌剂,Journal of Agricultural and Food Chemistry

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发现Frenolicin B作为控制小麦镰刀菌病的潜在农用化学杀菌剂
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-02-15 , DOI: 10.1021/acs.jafc.0c04277
Chuanyu Han ₁ , Zhiyin Yu _{1,

2} , Yuting Zhang ₁ , Zhiyan Wang ₂ , Junwei Zhao ₁ , Sheng-Xiong Huang ₂ , Zhonghua Ma ₃ , Ziyue Wen ₃ , Chongxi Liu _{1,

2} , Wensheng Xiang _{1,

4}

Affiliation

在这项研究中，从链霉菌的发酵液中提取上清液。NEAU-H3在体外和体内均显示出对禾谷镰孢菌株PH-1的强抗真菌活性。通过活性导向方法分离了三种已知的吡喃萘甲醌，并以特征为主要成分的果胶素B为特征。Frenolicin B对禾本科镰刀菌PH-1表现出较强的抗真菌活性，EC ₅₀值为0.51 mg / L，低于多菌灵（0.78 mg / L），但高于芬那克（0.18 mg / L）。。Frenolicin B还可以强烈抑制镰刀菌种的菌丝生长，包括从野外分离到的禾谷镰刀菌和亚洲镰刀菌以及耐多菌灵的镰刀菌菌株，其EC ₅₀值为0.25–0.92 mg / L。田间试验的结果表明，在浓度为75 g ai / ha的处理浓度下，苯酚菌素B在控制镰刀菌病中的功效要优于苯那可尔（375 g ai / ha）和多菌灵（600 g ai / ha）或具有与苯那可尔（375 g ai / ha）在2年内无显着差异。扫描电子显微镜和透射电子显微镜观察表明，治疗后禾谷镰刀菌菌丝体含有Fenolicin B，菌丝体异常，厚度和肿胀不均，细胞质崩解，部分细胞丢失。转录组分析表明，果胶蛋白B可能通过影响参与磷利用的基因来抑制核苷酸的代谢和能量，但不影响作为苯那克利的特定靶点的肌球蛋白5的表达。这些发现表明，贝那霉素B可能是控制镰刀菌枯萎病的潜在农药。

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Discovery of Frenolicin B as Potential Agrochemical Fungicide for Controlling Fusarium Head Blight on Wheat

In this study, the supernatant extract from fermentation broth of Streptomyces sp. NEAU-H3 showed strong antifungal activity against Fusarium graminearum strain PH-1 in vitro and in vivo. Three known pyranonaphthoquinones were isolated by means of an activity-guided method, and frenolicin B was characterized as the main active ingredient. Frenolicin B displayed strong antifungal activity against F. graminearum strain PH-1 with an EC₅₀ value of 0.51 mg/L, which is lower than that of carbendazim (0.78 mg/L) but higher than that of phenamacril (0.18 mg/L). Frenolicin B could also strongly inhibit the mycelial growth of Fusarium species, including F. graminearum and F. asiaticum, as well as carbendazim-resistant Fusarium strains isolated from field, with EC₅₀ values of 0.25–0.92 mg/L. Results from field experiments showed that the efficacy of frenolicin B in controlling Fusarium head blight at a treatment concentration of 75 g ai/ha was better than those of phenamacril (375 g ai/ha) and carbendazim (600 g ai/ha) or had no significant difference with that of phenamacril (375 g ai/ha) in 2 years. Scanning electron microscope and transmission electron microscope observations revealed that after treating F. graminearum mycelia with frenolicin B, the mycelia appeared aberrant and had an uneven thickness and swelling, the cytoplasm had disintegrated, and some cell contents were lost. Transcriptome analysis suggests that frenolicin B might inhibit the metabolism of nucleotides and energy by affecting genes involved in phosphorus utilization but did not affect the expression of myosin 5, which is the specific target of phenamacril. These findings indicate that frenolicin B may be a potential agrochemical fungicide for controlling Fusarium head blight.

更新日期：2021-02-24

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