Characterization of trans-Nerolidol Synthase from Celastrus angulatus Maxim and Production of trans-Nerolidol in Engineered Saccharomyces cerevisiae,Journal of Agricultural and Food Chemistry

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Characterization of trans-Nerolidol Synthase from Celastrus angulatus Maxim and Production of trans-Nerolidol in Engineered Saccharomyces cerevisiae
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-02-15 , DOI: 10.1021/acs.jafc.0c06084
Weiguo Li _{1,

2,

3} , Xiaoguang Yan _{1,

2,

3} , Yuting Zhang _{1,

2,

3} , Dongmei Liang _{1,

2,

3} , Qinggele Caiyin _{1,

2,

3} , Jianjun Qiao _{1,

2,

3,

4}

Affiliation

Volatile terpenoids are a large group of important secondary metabolites and possess many biological activities. The acyclic sesquiterpene trans-nerolidol is one of the typical representatives and widely used in cosmetics and agriculture. Here, the accumulation of volatile terpenes in different tissues of Celastrus angulatus was investigated, and two trans-nerolidol synthases, CaNES1 and CaNES2, were identified and characterized by in vitro enzymatic assays. Both genes are differentially transcribed in different tissues of C. angulatus. Next, we constructed a Saccharomyces cerevisiae cell factory to enable high-level production of trans-nerolidol. Glucose was the sole carbon source to sequentially control gene expression between the competitive squalene and trans-nerolidol pathways. Finally, the trans-nerolidol production of recombinant strain LWG003-CaNES2 was 7.01 g/L by fed-batch fermentation in a 5 L bioreactor. The results clarify volatile terpenoid biosynthesis in C. angulatus and provide a promising potential for industrial production of trans-nerolidol in S. cerevisiae.

中文翻译：

表征跨从-Nerolidol合酶苦皮藤马克西姆和生产反式在工程-Nerolidol酿酒酵母

挥发性萜类化合物是一大类重要的次生代谢产物，并具有许多生物学活性。无环倍半萜烯反式橙花醇是典型代表之一，广泛用于化妆品和农业。在这里，调查了Celastrus angulatus不同组织中挥发性萜烯的积累，并通过体外酶促测定鉴定和表征了两种反式橙花醇合酶CaNES1和CaNES2 。这两个基因在Angulatus的不同组织中差异转录。接下来，我们建立了酿酒酵母细胞工厂，以实现反式-nerolidol。葡萄糖是依次控制竞争角鲨烯和反式橙花醇途径之间基因表达的唯一碳源。最后，通过在5 L生物反应器中分批补料发酵，重组菌株LWG003- CaNES2的反式橙花醇产量为7.01 g / L。该结果阐明了角C中挥发性萜类的生物合成，并为啤酒酵母中反式橙花醇的工业生产提供了有希望的潜力。

更新日期：2021-02-24

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