Efficient hemostasis is of great significance in clinical and medical fields. Herein, inspired by the hierarchical microstructure of feet or stings of insects, we present a dodecyl-modified chitosan (DCS)-coated pagoda-like multilayer microneedle patch for tissue fixation and rapid hemostasis. Such a microneedle patch is fabricated by a step-by-step mold replication, which is easy-to-operate, flexible, and highly adjustable. The multilayer structure enables the microneedle patch to strongly fix onto diverse tissues via physical interlocking and is not affected by large blood loss or other conditions; while the DCS coating can anchor onto the cell membrane, bring about blood cell coagulation, and thus actively promote hemostasis. It was demonstrated from in-vivo rabbit experiments that acute tissue injuries including liver bleeding, spleen bleeding, and kidney bleeding can be repaired immediately by employing these DCS-coated multilayer microneedle patches. These distinctive features indicate that the presented microneedle patches can find applications in hemostasis and many other biomedical fields.

有效的止血在临床和医学领域具有重要意义。在此，受脚或昆虫st的分级微观结构的启发，我们提出了十二烷基修饰的壳聚糖（DCS）涂层宝塔状多层微针贴片，用于组织固定和快速止血。这种微针贴片是通过分步的模具复制制造的，该模具易于操作，灵活且高度可调。多层结构使微针贴片可以通过物理互锁牢固地固定在各种组织上，并且不受大量失血或其他情况的影响。DCS涂层可以锚定在细胞膜上，引起血细胞凝结，从而积极促进止血。活体内兔实验证明，急性组织损伤包括肝出血，脾脏出血，通过使用这些DCS涂层的多层微针贴片，可以立即修复肾脏出血。这些独特的特征表明，提出的微针贴片可以在止血和许多其他生物医学领域中找到应用。