Our study aimed at development of Silver, Iron and Gold nanoparticles of Lycopene isolated from tomato by using green synthesis technique and to evaluate its anticancer potential against colorectal and cervical cancer. Lycopene was extracted by benzene extraction method and the silver, iron and gold nanoparticles were developed by green synthesis method. 1% aqueous extract of isolated Lycopene was mixed with 1% solutions of AgNO₃, FeCl₃ and HAuCl₄ solutions and incubated at ambient temperature for 3–4 h separately and observed for the color change which is an indicative of formation of the nanoparticles. The prepared nanoparticles were characterized by FTIR, SEM, XRD analysis and evaluated for their antimicrobial potential. The cytotoxicity studies were carried out by in vitro assay like MTT, SRB and Tryphan blue method against Colo 320 DM, HT 29, and Hella. SEM showed nanosized particles of 50–100 nm range, whereas no antimicrobial activity was exhibited by the prepared nanoparticles. In MTT assay the LyAgNP showed maximum 41.41 ± 0.4124% inhibition against COLO320DM, whereas LyGNP exhibited 41.47 ± 0.4469% inhibition against HT 29 and LyAgNP showed 40.9 ± 0.6908% inhibition against Hella cells. In SRB assay LyAgNP showed maximum 82.68 ± 1.1798% inhibition against COLO320DM, whereas LyGNP exhibited maximum 91.21 ± 0.2372% inhibition against HT29 and 87.98 ± 0.5878% inhibition against Hella cells. In tryphan blue assay against COLO320DM, HT29 and Hella cells, the maximum inhibition exhibited by the prepared nanoparticles were observed as LyGNP 83.45 ± 0.4694%, LyAgNP 88.05 ± 0.1870% and LyAgNP65.47 ± 0.4766%. We conclude that the developed nanoparticles of Lycopene exhibited potential anticancer activity against Colorectal and cervical cancer cell as compared with pure Lycopene.

我们的研究旨在通过绿色合成技术开发从番茄中分离的番茄红素银、铁和金纳米颗粒，并评估其对结直肠癌和宫颈癌的抗癌潜力。采用苯萃取法提取番茄红素，采用绿色合成法开发银、铁、金纳米颗粒。将分离的番茄红素的 1% 水提物与 1% 的 AgNO ₃、FeCl ₃和 HAuCl ₄溶液混合溶液并在环境温度下分别孵育 3-4 小时，观察颜色变化，这表明纳米颗粒的形成。制备的纳米颗粒通过 FTIR、SEM、XRD 分析进行表征并评估其抗菌潜力。细胞毒性研究是通过体外试验如 MTT、SRB 和色氨酸蓝方法对 Colo 320 DM、HT 29 和 Hella 进行的。SEM 显示纳米颗粒范围为 50-100 nm，而制备的纳米颗粒没有表现出抗菌活性。在 MTT 测定中，LyAgNP 对 COLO320DM 的抑制作用最大为 41.41 ± 0.4124%，而 LyGNP 对 HT 29 的抑制作用为 41.47 ± 0.4469%，而 LyAgNP 对海拉细胞的抑制作用为 40.9 ± 0.6908%。在 SRB 测定中，LyAgNP 显示对 COLO320DM 的最大抑制率为 82.68 ± 1.1798%，而 LyGNP 对 HT29 的抑制作用最大为 91.21 ± 0.2372%，对海拉细胞的抑制作用最大为 87.98 ± 0.5878%。在针对 COLO320DM、HT29 和 Hella 细胞的色氨酸蓝测定中，观察到制备的纳米颗粒表现出的最大抑制为 LyGNP 83.45 ± 0.4694%、LyAgNP 88.05 ± 0.1870% 和 LyAgNP65.47 ± 0.4766%。我们得出结论，与纯番茄红素相比，开发的番茄红素纳米粒子对结直肠癌和宫颈癌细胞具有潜在的抗癌活性。