Nanoparticle-Mediated Assembly of Peptoid Nanosheets Functionalized with Solid-Binding Proteins: Designing Heterostructures for Hierarchy,Nano Letters

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Nanoparticle-Mediated Assembly of Peptoid Nanosheets Functionalized with Solid-Binding Proteins: Designing Heterostructures for Hierarchy
Nano Letters ( IF 9.6 ) Pub Date : 2021-02-08 , DOI: 10.1021/acs.nanolett.0c04285
Jinrong Ma , Bin Cai ₁ , Shuai Zhang ₁ , Tengyue Jian ₁ , James J De Yoreo ₁ , Chun-Long Chen ₁ , François Baneyx

Affiliation

The fabrication of ordered architectures that intimately integrate polymer, protein, and inorganic components remains difficult. Two promising building blocks to tackle this challenge are peptoids, peptide mimics capable of self-assembly into well-defined structures, and solid-binding peptides, which offer a biological path to controlled inorganic assembly. Here, we report on the synthesis of 3.3-nm-thick thiol-reactive peptoid nanosheets from equimolar mixtures of unmodified and maleimide-derivatized versions of the Nbpe₆Nce₆ oligomer, optimize the location of engineered cysteine residues in silica-binding derivatives of superfolder green fluorescent protein for maleimide conjugation, and react the two components to form protein–peptoid hybrids exhibiting partial or uniform protein coverage on both of their sides. Using 10 nm silica nanoparticles, we trigger the stacking of these 2D structures into a multilayered material composed of alternating peptoid, protein, and organic layers. This simple and modular approach to hierarchical hybrid synthesis should prove useful in bioimaging and photocatalysis applications.

中文翻译：

纳米粒子介导的类固醇结合蛋白功能化的类肽纳米片的组装：设计层次结构的异质结构。

紧密集成聚合物，蛋白质和无机成分的有序体系结构的制造仍然很困难。应对这一挑战的两个有希望的构建基块是拟肽，能够自组装成明确结构的肽模拟物和固体结合肽，它们为控制无机组装提供了生物学途径。在这里，我们报道由未改性和马来酰亚胺衍生的Nbpe ₆ Nce _6的等摩尔混合物合成3.3 nm厚的巯基反应性拟肽纳米片。寡聚体，优化工程改造的半胱氨酸残基在超级文件夹绿色荧光蛋白的二氧化硅结合衍生物中的位置，以进行马来酰亚胺偶联，并使这两种组分反应形成蛋白-类肽杂化物，在两侧均具有部分或均匀的蛋白覆盖。使用10 nm二氧化硅纳米颗粒，我们触发了将这些2D结构堆叠到由交替的类肽，蛋白质和有机层组成的多层材料中。这种简单的，模块化的分层混合合成方法在生物成像和光催化应用中应被证明是有用的。

更新日期：2021-02-24

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