Daturataturin A (DTA), a withanolide compound in Datura metel L., exhibits excellent anti‐inflammatory and anti‐proliferative activities. Here, we report the study of DTA‐induced proliferation and inflammation in human immortalized keratinocytes (HaCaTs) and the associated molecular mechanisms. HaCaTs are a model of the epidermal proliferative state of cells. The pharmacodynamics and mechanism of DTA were studied by western blot, immunofluorescence, apoptosis and proliferation detection, and real‐time quantitative polymerase chain reaction. We confirmed that DTA induced HaCaT autophagy, which, in turn, induced HaCaT senescence and, ultimately, led to cell cycle arrest. DTA also negatively regulated inflammation through the activation of autophagy. This may be one of the mechanisms underlying the action of Datura metel L. preparation used for the treatment of psoriasis.

中文翻译：

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Datura metel L.中的一种醇化物Daturataturin A通过PI3K-Akt-mTOR信号传导途径诱导HaCaT自噬

Datura metel L.中的一种醇化物Daturataturin A（DTA）具有出色的抗炎和抗增殖活性。在这里，我们报告了DTA诱导的人类永生化角质形成细胞（HaCaTs）增殖和炎症及其相关分子机制的研究。HaCaT是细胞表皮增殖状态的模型。通过蛋白质印迹，免疫荧光，凋亡和增殖检测以及实时定量聚合酶链反应研究了DTA的药效学和机理。我们证实DTA诱导了HaCaT自噬，进而引起HaCaT衰老，并最终导致细胞周期停滞。DTA还通过自噬激活来消极调节炎症。这可能是潜在的行动机制之一用于治疗牛皮癣的曼陀罗梅特尔乳杆菌制剂。