基于细胞代谢组学方法揭示传代数对HT29细胞系的影响,Journal of Proteome Research

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基于细胞代谢组学方法揭示传代数对HT29细胞系的影响
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2021-02-08 , DOI: 10.1021/acs.jproteome.0c00806
Jianhua Cao ₁ , Xingkang Wu ₁ , Xuemei Qin ₁ , Zhenyu Li ₁

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在设计基于细胞系的实验时，传代数是重要的因素。尽管HT29细胞已在实验室中广泛用于结直肠癌研究，但细胞传代数对HT29细胞的影响仍然未知。在这项研究中，表型测定和代谢组学方法被用于分析传代数（传代4、10和16）对HT29细胞的全身作用。结果表明，增加的细胞传代数影响了细胞周期分布，也降低了HT29细胞的增殖和迁移能力。代谢组学分析，热图分析和层次聚类分析表明，不同代数的细胞之间存在明显的代谢组差异，这与61种不同的代谢产物有关。确定了三种代谢途径作为关键途径，精氨酸参与了其中的两个。另外，发现精氨酸补充可以抑制HT29细胞的体外增殖能力，并且精氨酸和顺铂之间存在协同作用。总之，这项研究不仅揭示了传代数对HT29细胞的影响，而且为精氨酸具有作为顺铂治疗佐剂的潜在作用提供了重要的参考。

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Uncovering the Effect of Passage Number on HT29 Cell Line Based on the Cell Metabolomic Approach

The passage number is an important factor when designing the cell line-based experiment. Although HT29 cells were widely used in the laboratory for colorectal cancer studies, the impact of cell passage number on the HT29 cells was still unknown. In this study, phenotypic assay and metabolomic approach were applied to analyze the systemic effects of passage numbers (passage 4, 10, and 16) on the HT29 cells. The results showed that the increased cell passage number affected the cell cycle distribution and also decreased the proliferation and migration ability of HT29 cells. The metabolomic analysis coupled with heatmap and hierarchical cluster analysis showed obvious metabolome difference among the cells with different passage numbers, which was related with 61 differential metabolites. Three metabolic pathways were determined as the key pathways, and arginine participated in two of them. In addition, it was found that arginine supplementation could inhibit the proliferation ability of HT29 cells in vitro, and a synergistic effect existed between arginine and cisplatin. In conclusion, this study not only revealed the influence of passage numbers on the HT29 cell but also provided an important reference that arginine has the potential role to be developed as the cisplatin therapeutic adjuvant.

更新日期：2021-03-05

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