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MnO2 shell-isolated SERS nanoprobe for the quantitative detection of ALP activity in trace serum: Relying on the enzyme-triggered etching of MnO2 shell to regulate the signal
Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2021-02-08 , DOI: 10.1016/j.snb.2021.129605
Xin Dai , Liangwei Lu , Xuanhao Zhang , Zhi-Ling Song , Wenjuan Song , Qiqi Chao , Qian Li , Wei Wang , Junfeng Chen , Gao-Chao Fan , Xiliang Luo

Establishing a stable, reliable and sensitive assay for the direct analysis in trace serum is of significance for the medical diagnosis and disease monitoring. We develop a MnO2 shell-isolated SERS nanoprobe (Au-Mpy-Au-MnO2, AMAM) for the quantitative detection of alkaline phosphatase (ALP) in trace serum. Due to the physical isolation of MnO2 shell, AMAM is equipped with high stability, rarely happening the accidental aggregation. Particularly, the SERS signal is regulated by the etching of MnO2 shell, requiring not to change the local electromagnetic field or the molecular structure of Raman reporter (4-mercaptopyridine (Mpy)). Designating MnO2 shell as the Raman reference and the ratio of IMpy/IMnO2 as the response signal, AMAM system can obviously eliminate the experimental interference and achieve highly reliable SERS quantification for ALP. This ratiometric SERS system exhibits good reproducibility, high stability and sensitivity for the ALP analysis. AMAM system is able to perform the colorimetric and SERS dual-readout detection, which displays a wide linear dynamic range (0.1–70 U/L) for ALP with a detection limit of 0.079 U/L. Additionally, integrating with in-capillary SERS technique, our proposed nanoprobe is also applicable of direct SERS analysis in trace undiluted human serum (2 μL).



中文翻译:

MnO 2壳分离的SERS纳米探针用于痕量血清中ALP活性的定量检测:依靠酶触发的MnO 2壳蚀刻来调节信号

建立稳定,可靠和灵敏的分析方法直接分析痕量血清对医学诊断和疾病监测具有重要意义。我们开发了一种MnO 2壳分离的SERS纳米探针(Au-Mpy-Au-MnO 2,AMAM),用于定量检测痕量血清中的碱性磷酸酶(ALP)。由于MnO 2壳的物理隔离,AMAM具有很高的稳定性,很少发生意外聚集。特别地,通过对MnO 2壳的蚀刻来调节SERS信号,不需要改变局部电磁场或拉曼报道分子(4-巯基吡啶(Mpy))的分子结构。指定MnO 2壳作为拉曼参考和I之比以Mpy / I MnO2作为响应信号,AMAM系统可以明显消除实验干扰,实现对ALP的高度可靠的SERS定量。这种比例SERS系统对ALP分析显示出良好的重现性,高稳定性和灵敏度。AMAM系统能够执行比色和SERS双重读数检测,这对ALP显示了宽的线性动态范围(0.1–70 U / L),检测极限为0.079 U / L。此外,与毛细管内SERS技术集成后,我们提出的纳米探针也可用于痕量未稀释人血清(2μL)中的直接SERS分析。

更新日期:2021-02-17
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