Bi nanoparticles (NPs) have been demonstrated as effective all-in-one type theranostic agent for imaging-guided photothermal therapy, but their applications have been limited by relatively low biocompatibility and target accumulation capacity. To address this issue, we report the camouflage of Bi NPs (size: ~42 ± 2 nm) by using the mouse colon cancer CT26 cells membrane (CT26 CCM). The camouflaging process confers the efficient coating of CCM shell layer with thickness of ~8 ± 2 nm on Bi NPs cores, which can be confirmed by TEM image, zeta potential and protein gel electrophoresis tests. Simultaneously, CCM shell has no side effects on the photoabsorption/photothermal effect. Importantly, Bi@CCM NPs retain significant features of CCM, including good biocompatibility and homologous targeting ability. When Bi@CCM dispersion was intravenously (i.v.) injected into mice, they exhibited higher blood circulation half-life (11.5 h, ~2.9 times) and accumulation amount (4.7 ± 0.56% ID/g, ~2.3 times) in homotypic CT26 tumor compared to those (4.0 h in blood and 2.03 ± 0.60% ID/g in tumor) from uncoated Bi NPs. After 808 nm laser irradiation, CT26 cancer cells could be effectively ablated after the photothermal therapy of high-accumulated Bi@CCM NPs, and then the tumor tends to be eradicated after 12 days. Thus, Bi NPs camouflaged with CT26 CCM have great potential for the targeted photothermal therapy of homotypic tumors.

Bi纳米粒子（NPs）已被证明是用于成像引导光热疗法的有效的多合一型治疗剂，但由于相对较低的生物相容性和靶标累积能力，它们的应用受到了限制。为了解决这个问题，我们报告了使用小鼠结肠癌CT26细胞膜（CT26 CCM）掩盖Bi NP（尺寸：〜42±2 nm）的现象。伪装过程可在Bi NPs核芯上有效涂覆CCM壳层，厚度约为〜8±2 nm，这可以通过TEM图像，ζ电位和蛋白质凝胶电泳测试来确认。同时，CCM壳对光吸收/光热效应没有副作用。重要的是，Bi @ CCM NP保留了CCM的重要功能，包括良好的生物相容性和同源靶向能力。当静脉注射Bi @ CCM分散液时（iv ）注射到小鼠体内后，同型CT26肿瘤的血液循环半衰期（11.5 h，〜2.9倍）和累积量（4.7±0.56％ID / g，〜2.3倍）高于血液（4.0 h来自未包被的Bi NP的2.03±0.60％ID / g）。808 nm激光照射后，光热治疗高积累的Bi @ CCM NPs可以有效消融CT26癌细胞，然后在12天后趋于根除。因此，被CT26 CCM伪装的Bi NP在同型肿瘤的靶向光热治疗中具有巨大潜力。808 nm激光照射后，光热治疗高积累的Bi @ CCM NPs可以有效消融CT26癌细胞，然后在12天后趋于根除。因此，被CT26 CCM伪装的Bi NP在同型肿瘤的靶向光热治疗中具有巨大潜力。808 nm激光照射后，光热治疗高积累的Bi @ CCM NPs可以有效消融CT26癌细胞，然后在12天后趋于根除。因此，被CT26 CCM伪装的Bi NP在同型肿瘤的靶向光热治疗中具有巨大潜力。