Wound management poses a considerable economic burden on the global healthcare system, considering the impacts of wound infection, delayed healing and scar formation. To this end, multifunctional dressings based on hydrogels have been developed to stimulate skin healing. Herein, we describe the design, fabrication, and characterization of a sprayable hydrogel-based wound dressing loaded with cerium oxide nanoparticles (CeONs) and an antimicrobial peptide (AMP), for combined reactive oxygen species (ROS)-scavenging and antibacterial properties. We adopted a mussel-inspired strategy to chemically conjugate gelatin with dopamine motifs and prepared a hydrogel dressing with improved binding affinity to wet skin surfaces. Additionally, the release of AMP from the hydrogel demonstrated rapid release ablation and contact ablation against four representative bacterial strains, confirming the desired antimicrobial activities. Moreover, the CeONs-loaded hydrogel dressing exhibited favorable ROS-scavenging abilities. The biocompatibility of the multifunctional hydrogel dressing was further proven in vitro by culturing with HaCaT cells. Overall, the benefits of the developed hydrogel wound dressing, including sprayability, adhesiveness, antimicrobial activity, as well as ROS-scavenging and skin-remodeling ability, highlight its promissing translational potentials in wound management.

Statement of Significance

Various hydrogel-based wound-dressing materials have been developed to stimulate wound healing. However, from the clinical perspective, few of the current wound dressings meet all the intended multifunctional requirements of preventing infection, promoting rapid wound closure, and minimizing scar formation, while simultaneously offering the convenience of application. In the current study, we adopted a mussel-inspired strategy to functionalize the GelMA hydrogels with DOPA to fabricate GelMA-DOPA hydrogel which exhibited an enhanced binding affinity for wound surfaces, AMP HHC-36 and CeONs are further encapsulated into the GelMA-DOPA hydrogel to confer the hydrogel wound dressing with antimicrobial and ROS-scavenging abilities. The GelMA-DOPA-AMP-CeONs dressing offered the benefits of sprayability, adhesiveness, antimicrobial activity, as well as ROS-scavenging and skin-remodeling ability, which might address the therapeutic and economic burdens associated with chronic wound treatment and management.

中文翻译：

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可喷涂的水凝胶敷料结合了活性氧清除和抗菌功能，可加速伤口愈合

考虑到伤口感染，延迟愈合和疤痕形成的影响，伤口管理给全球医疗系统带来了巨大的经济负担。为此，已经开发了基于水凝胶的多功能敷料以刺激皮肤愈合。在本文中，我们描述了负载有氧化铈纳米颗粒（CeONs）和抗菌肽（AMP）的可喷雾水凝胶基伤口敷料的设计，制造和表征，用于组合的活性氧（ROS）清除和抗菌性能。我们采用了以贻贝为灵感的策略，将明胶与多巴胺基序化学偶联，并制备了具有改善的对湿润皮肤表面的结合亲和力的水凝胶敷料。此外，AMP从水凝胶中的释放证明了针对四种代表性细菌菌株的快速释放消融和接触消融，从而确认了所需的抗菌活性。此外，负载CeONs的水凝胶敷料具有良好的ROS清除能力。多功能水凝胶敷料的生物相容性得到进一步证明通过与HaCaT细胞一起体外培养。总体而言，开发的水凝胶伤口敷料的优势包括可喷涂性，粘合性，抗菌活性以及ROS清除能力和皮肤重塑能力，突出了其在伤口管理中的潜在转化潜力。

重要声明

已经开发了各种基于水凝胶的伤口敷料来刺激伤口愈合。但是，从临床角度来看，当前的伤口敷料很少能满足所有预期的多功能性要求，即防止感染，促进伤口快速闭合并最大程度地减少疤痕形成，同时又提供了使用的便利性。在当前的研究中，我们采用了贻贝启发的策略，用DOPA对GelMA水凝胶进行功能化，以制造对伤口表面表现出增强的结合亲和力的GelMA-DOPA水凝胶，AMP HHC-36和CeON被进一步封装到GelMA-DOPA水凝胶中使水凝胶伤口敷料具有抗菌和清除ROS的能力。GelMA-DOPA-AMP-CeONs敷料具有可喷涂性，粘合性，抗菌活性，