Surface-Directed Mineralization of Fibrous Collagen Scaffolds in Simulated Body Fluid for Tissue Engineering Applications,ACS Applied Bio Materials

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Surface-Directed Mineralization of Fibrous Collagen Scaffolds in Simulated Body Fluid for Tissue Engineering Applications
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2021-02-05 , DOI: 10.1021/acsabm.0c01507
Odair Bim-Júnior _{1,

2} , Fabiana Curylofo-Zotti _{1,

3} , Mariana Reis ₁ , Yvette Alania ₁ , Paulo N Lisboa-Filho ₂ , Ana K Bedran-Russo ₁

Affiliation

The use of polymer additives that stabilize fluidic amorphous calcium phosphate is key to obtaining intrafibrillar mineralization of collagen in vitro. On the other hand, this biomimetic approach inhibits the nucleation of mineral crystals in unconfined extrafibrillar spaces, that is, extrafibrillar mineralization. The extrafibrillar mineral content is a significant feature to replicate from hard connective tissues such as bone and dentin as it contributes to the final microarchitecture and mechanical stiffness of the biomineral composite. Herein, we report a straightforward route to produce densely mineralized collagenous composites via a surface-directed process devoid of the aid of polymer additives. Simulated body fluid (1×) is employed as a biomimetic crystallizing medium, following a preloading procedure on the collagen surface to quickly generate the amorphous precursor species required to initiate matrix mineralization. This approach consistently leads to the formation of extrafibrillar bioactive minerals in bulk collagen scaffolds, which may offer an advantage in the production of osteoconductive collagen–apatite materials for tissue engineering and repair purposes.

中文翻译：

用于组织工程应用的模拟体液中纤维状胶原支架的表面定向矿化

使用稳定流体无定形磷酸钙的聚合物添加剂是体外获得胶原蛋白纤维内矿化的关键。另一方面，这种仿生方法抑制了无限制的纤维外空间中矿物晶体的成核，即纤维外矿化。纤维外矿物质含量是从硬结缔组织（如骨和牙本质）复制的重要特征，因为它有助于生物矿物复合材料的最终微结构和机械刚度。在这里，我们报告了一种直接的途径，通过没有聚合物添加剂帮助的表面导向工艺生产致密矿化胶原复合材料。模拟体液（1×）用作仿生结晶介质，在胶原表面上进行预加载程序以快速生成启动基质矿化所需的无定形前体物质。这种方法始终导致在大块胶原支架中形成纤维外生物活性矿物质，这可能在生产用于组织工程和修复目的的骨传导性胶原-磷灰石材料方面具有优势。

更新日期：2021-03-15

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