RNA interference is an important method in studying the gene function of organisms. As a novel important gene in gonadal development of teleost fish, the function of igf3 is worth of researching. In this study, the igf3b-dsRNA of C. carpio was successfully synthesized, and long-term igf3 interference experiments were conducted. Intraperitoneal injection of igf3b-dsRNA could influence the serum sex hormone level and significantly reduced the expression level of insulin-like growth factors (IGFs) in hypothalamic–pituitary–gonad (HPG) axis. After a 60-day interference experiment, igf3 knockdown could affect the development of ovary and testis of juvenile C. carpio. These findings show that igf3 knockdown may have a negative effect on gonadal development of C. carpio through HPG axis.

中文翻译：

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通过RNA干扰敲除igf3基因导致鲤鱼（Cyprinus carpio）的性腺发育抑制

RNA干扰是研究生物体基因功能的重要方法。作为硬骨鱼性腺发育的一个新的重要基因，igf3的功能值得研究。在这项研究中，igf3b的-dsRNA鲤鱼被成功合成，长期igf3进行了干扰实验。腹腔注射igf3b- dsRNA可影响血清性激素水平，并显着降低下丘脑-垂体-性腺（HPG）轴中胰岛素样生长因子（IGF）的表达水平。经过60天的干扰实验，敲除igf3可能会影响C. carpio幼鱼卵巢和睾丸的发育. 这些发现表明igf3敲低可能通过 HPG 轴对C. carpio 的性腺发育产生负面影响。