用于软骨重建的组织粘附硫酸软骨素水凝胶,ACS Biomaterials Science & Engineering

当前位置： X-MOL 学术 › ACS Biomater. Sci. Eng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

用于软骨重建的组织粘附硫酸软骨素水凝胶
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2021-02-04 , DOI: 10.1021/acsbiomaterials.0c01414
Jisoo Shin ₁ , Eun Hye Kang ₂ , Soojeong Choi ₁ , Eun Je Jeon ₁ , Jung Ho Cho ₁ , Donyoung Kang ₃ , Hyungsuk Lee ₃ , In Sik Yun ₄ , Seung-Woo Cho _{1,

5,

6}

Affiliation

硫酸软骨素（CS）是软骨细胞外基质的主要成分，作为软骨组织工程的生物材料引起了人们的关注。然而，目前的 CS 水凝胶系统在成功的软骨组织工程中仍然存在局限性，因为它们的降解动力学不合适、机械相似性不足以及与天然软骨组织缺乏整合。在这项研究中，我们使用受贻贝粘合剂启发的儿茶酚化学，开发了一种功能性 CS 水凝胶，该水凝胶具有可调的物理和机械性能以及出色的组织粘附力，可与天然组织有效整合。开发的儿茶酚功能化 CS (CS-CA) 水凝胶的各种性能，包括溶胀、降解、机械性能和粘附性，可以通过改变儿茶酚基团与 CS 骨架的缀合比率和 CS-CA 缀合物的浓度来定制。与传统的 CS 水凝胶（~数百 Pa 和~0.05 N）相比，CS-CA 水凝胶表现出显着增加的模量（~10 kPa）和优异的粘合性能（~3 N）。此外，结合脱细胞软骨组织骰子的 CS-CA 水凝胶通过提供软骨样微环境促进人脂肪来源间充质干细胞的软骨分化。最后，由于有利于软骨形成的物理、机械和生物学特性，使用组织粘附性 CS-CA 水凝胶移植自体软骨切片增强了软骨与宿主组织的整合和新软骨的形成。综上所述，

"点击查看英文标题和摘要"

Tissue-Adhesive Chondroitin Sulfate Hydrogel for Cartilage Reconstruction

Chondroitin sulfate (CS), the main component of cartilage extracellular matrix, has attracted attention as a biomaterial for cartilage tissue engineering. However, current CS hydrogel systems still have limitations for application in successful cartilage tissue engineering owing to their unsuitable degradation kinetics, insufficient mechanical similarity, and lack of integration with the native cartilage tissue. In this study, using mussel adhesive-inspired catechol chemistry, we developed a functional CS hydrogel that exhibits tunable physical and mechanical properties as well as excellent tissue adhesion for efficient integration with native tissues. Various properties of the developed catechol-functionalized CS (CS-CA) hydrogel, including swelling, degradation, mechanical properties, and adhesiveness, could be tailored by varying the conjugation ratio of the catechol group to the CS backbone and the concentration of the CS-CA conjugates. CS-CA hydrogels exhibited significantly increased modulus (∼10 kPa) and superior adhesive properties (∼3 N) over conventional CS hydrogels (∼hundreds Pa and ∼0.05 N). In addition, CS-CA hydrogels incorporating decellularized cartilage tissue dice promoted the chondrogenic differentiation of human adipose-derived mesenchymal stem cells by providing a cartilage-like microenvironment. Finally, the transplantation of autologous cartilage dice using tissue-adhesive CS-CA hydrogels enhanced cartilage integration with host tissue and neo-cartilage formation owing to favorable physical, mechanical, and biological properties for cartilage formation. In conclusion, our study demonstrated the potential utility of the CS-CA hydrogel system in cartilage tissue reconstruction.

更新日期：2021-02-04

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南