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Purification of Gekko Small Peptide Fraction and Its Effect of Inducing Apoptosis of EC 9706 Esophageal Cancer Cells by Inhibiting PI3K/Akt/GLUT1 Signaling Pathway
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2021-02-03 , DOI: 10.1002/cbdv.202000720
Xingzhi Lv 1 , Ruifang Li 1 , Zhongjie Li 1 , Jiangang Wang 1
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2021-02-03 , DOI: 10.1002/cbdv.202000720
Xingzhi Lv 1 , Ruifang Li 1 , Zhongjie Li 1 , Jiangang Wang 1
Affiliation
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This study aimed to isolate and purify a cytotoxic extraction from Gekko japonicus, identify its components and determine its cytotoxic activity in vitro. We isolated and identified the most potent cytotoxic Gekko small peptide LH‐20‐15. The identification and analysis of peptide sequences of LH‐20‐15 were performed by de novo peptide sequencing, and two new peptides were found. LH‐20‐15 significantly inhibited the proliferation of human esophageal squamous carcinoma EC 9706 cells in a dose‐dependent manner. Furthermore, LH‐20‐15 induced apoptosis in esophageal cancer cells by activating the mitochondrial apoptotic pathway. Further research showed that LH‐20‐15 inhibited the PI3 K/Akt/GLUT1 signaling pathway. In conclusion, LH‐20‐15 from Gekko japonicus is a peptide mixture and may inhibit EC 9706 cell proliferation and induce apoptosis by activating the mitochondrial apoptotic pathway. It also regulates glucose metabolism by targeting the PI3 K/Akt/GLUT1 signaling pathway. These small peptides could be new sources of natural cytotoxic ingredients against esophageal cancer with potential drug values.
中文翻译：
Gekko小肽片段的纯化及其通过抑制PI3K / Akt / GLUT1信号通路诱导EC 9706食管癌细胞凋亡的作用
这项研究的目的是分离和纯化日本壁虎的细胞毒性提取物，鉴定其成分并确定其在体外的细胞毒性活性。我们分离并鉴定出最有效的细胞毒性Gekko小肽LH-20-15。通过从头肽测序对LH-20-15的肽序列进行鉴定和分析，发现了两个新的肽。LH-20-15以剂量依赖性方式显着抑制人食道鳞状细胞癌EC 9706细胞的增殖。此外，LH-20-15通过激活线粒体凋亡途径诱导食道癌细胞凋亡。进一步的研究表明，LH-20-15抑制了PI3 K / Akt / GLUT1信号通路。总之，来自Gekko japonicus是一种肽混合物，可通过激活线粒体凋亡途径来抑制EC 9706细胞增殖并诱导凋亡。它还通过靶向PI3 K / Akt / GLUT1信号通路来调节葡萄糖代谢。这些小肽可能是抗食管癌的具有潜在药物价值的天然细胞毒性成分的新来源。
更新日期：2021-03-11
中文翻译：
Gekko小肽片段的纯化及其通过抑制PI3K / Akt / GLUT1信号通路诱导EC 9706食管癌细胞凋亡的作用
这项研究的目的是分离和纯化日本壁虎的细胞毒性提取物，鉴定其成分并确定其在体外的细胞毒性活性。我们分离并鉴定出最有效的细胞毒性Gekko小肽LH-20-15。通过从头肽测序对LH-20-15的肽序列进行鉴定和分析，发现了两个新的肽。LH-20-15以剂量依赖性方式显着抑制人食道鳞状细胞癌EC 9706细胞的增殖。此外，LH-20-15通过激活线粒体凋亡途径诱导食道癌细胞凋亡。进一步的研究表明，LH-20-15抑制了PI3 K / Akt / GLUT1信号通路。总之，来自Gekko japonicus是一种肽混合物，可通过激活线粒体凋亡途径来抑制EC 9706细胞增殖并诱导凋亡。它还通过靶向PI3 K / Akt / GLUT1信号通路来调节葡萄糖代谢。这些小肽可能是抗食管癌的具有潜在药物价值的天然细胞毒性成分的新来源。
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