<br>氧梯度和培养条件对间充质干细胞命运的影响差异,Advanced Healthcare Materials

当前位置： X-MOL 学术 › Adv. Healthcare Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

氧梯度和培养条件对间充质干细胞命运的影响差异
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2021-02-02 , DOI: 10.1002/adhm.202002058
Jay R. K. Samal ₁ , Vignesh K. Rangasami ₂ , Sumanta Samanta ₂ , Oommen P. Varghese ₃ , Oommen P. Oommen ₂

Affiliation

在过去的几年里，间充质干细胞（或基质）细胞（MSC）因其治疗价值而引起了极大的兴趣，尤其是其多系分化潜力导致了再生医学的应用。MSC 在体外扩增时发生生理变化，导致不同受体的表达，从而诱导治疗效果的高度可变性。因此，了解影响这些细胞分化或增殖的天然局部信号的生化线索非常重要。有几篇报道表明，在低氧梯度（或低氧条件）下体外培养 MSC 会上调干性标志物并促进未分化状态下的细胞增殖，因为缺氧模拟了祖细胞在组织内经历的条件。然而，不同的研究报告了不同的氧梯度和培养条件，导致他们对结果的解释存在歧义。在本进展报告中，旨在总结该领域的最新研究，特别关注在应用低氧条件以改善 MSC 增殖或分化期间报告的相互矛盾的结果。此外，它试图破译在缺氧下可能影响 MSC 特征的因素，并提出了一些可以与缺氧细胞培养相结合以更好地概括 MSC 组织生态位的技术。

"点击查看英文标题和摘要"

Discrepancies on the Role of Oxygen Gradient and Culture Condition on Mesenchymal Stem Cell Fate

Over the past few years, mesenchymal stem (or stromal) cells (MSCs) have garnered enormous interest due to their therapeutic value especially for their multilineage differentiation potential leading to regenerative medicine applications. MSCs undergo physiological changes upon in vitro expansion resulting in expression of different receptors, thereby inducing high variabilities in therapeutic efficacy. Therefore, understanding the biochemical cues that influence the native local signals on differentiation or proliferation of these cells is very important. There have been several reports that in vitro culture of MSCs in low oxygen gradient (or hypoxic conditions) upregulates the stemness markers and promotes cell proliferation in an undifferentiated state, as hypoxia mimics the conditions the progenitor cells experience within the tissue. However, different studies report different oxygen gradients and culture conditions causing ambiguity in their interpretation of the results. In this progress report, it is aimed to summarize recent studies in the field with specific focus on conflicting results reported during the application of hypoxic conditions for improving the proliferation or differentiation of MSCs. Further, it is tried to decipher the factors that can affect characteristics of MSC under hypoxia and suggest a few techniques that could be combined with hypoxic cell culture to better recapitulate the MSC tissue niche.

更新日期：2021-03-17

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南