根际微生物是植物抗逆性的重要伙伴。最近的研究表明,丛枝菌根真菌(AMF)与根际微生物组相互作用,可以促进被重金属污染的土壤的恢复。但是,尚不清楚AMF如何影响根际微生物组,以改善稀土元素(REEs)胁迫下的植物生长。将AMF(Claroideoglomus etunicatum)接种到掺有镧(0 mg kg -1,La0; 10 mg kg -1,La10; 100 mg kg -1,La100; 500 mg kg -1)的土壤中生长的玉米上。,La500)。评价了植物生物量,养分吸收,稀土元素吸收以及根际细菌和真菌群落。结果表明,La100和La500显着降低了根部定植率和营养吸收(钾,磷,钙和镁含量)。La500显着降低了细菌和真菌群落的α多样性指数。在所有La处理中,AMF显着提高了玉米的地上部和根鲜重和干重(La0和La10处理的根鲜重和干重除外)。对于La100和La500处理,AMF使玉米芽中的营养吸收(钾,磷,钙和镁含量)显着提高27.40–441.77%。对于La500处理,AMF使玉米中的地上部La浓度显着降低51.53%,但使根部La浓度显着提高30.45%。此外,AMF在La0处理中降低了细菌和真菌的Shannon指数,但是在La500处理中增加了细菌Shannon指数。AMF和La500均显着影响细菌和真菌的群落组成,并且AMF比La引起的影响更大。AMF促进了细菌的富集,包括La500中的疟原虫,溶杆菌,酿酒酵母,农杆菌,微细菌,链霉菌,青霉属和其他未分类属,以及真菌(青霉属)具有促进植物生长和对重金属的耐受性的功能。研究表明,AMF可以调节根际细菌和真菌的成分,并培育某些有益微生物,以增强La胁迫下玉米的耐性。AMF辅助的植物修复是一种改善被REEs污染土壤的诱人方法。
"点击查看英文标题和摘要"
Arbuscular mycorrhizal fungi alter microbiome structure of rhizosphere soil to enhance maize tolerance to La
Rhizosphere microbes are essential partners for plant stress tolerance. Recent studies indicate that arbuscular mycorrhizal fungi (AMF) can facilitate the revegetation of soils contaminated by heavy metals though interacting with rhizosphere microbiome. However, it is unclear how AMF affect rhizosphere microbiome to improve the growth of plant under rare earth elements (REEs) stress. AMF (Claroideoglomus etunicatum) was inoculated to maize grown in soils spiked with Lanthanum (0 mg kg−1, La0; 10 mg kg−1, La10; 100 mg kg−1, La100; 500 mg kg−1, La500). Plant biomass, nutrient uptake, REE uptake and rhizosphere bacterial and fungal community were evaluated. The results indicated that La100 and La500 decreased significantly root colonization rates and nutrition uptake (K, P, Ca and Mg content). La500 decreased significantly α-diversity indexes of bacterial and fungal community. AMF enhanced significantly the shoot and root fresh and dry weight of maize in all La treatments (except for the root fresh and dry weight of La0 and La10 treatment). For La100 and La500 treatments, AMF increased significantly nutrition uptake (K, P, Ca and Mg content) in shoot of maize by 27.40–441.77%. For La500 treatment, AMF decreased significantly shoot La concentration by 51.53% in maize, but increased significantly root La concentration by 30.45%. In addition, AMF decreased bacterial and fungal Shannon index in La0 treatment, but increased bacterial Shannon index in La500 treatment. Both AMF and La500 affected significantly the bacterial and fungal community composition, and AMF led to more influence than La. AMF promoted the enrichment of bacteria, including Planomicrobium, Lysobacter, Saccharothrix, Agrococcus, Microbacterium, Streptomyces, Penicillium and other unclassified genus, and fungi (Penicillium) in La500, which showed the function for promoting plant growth and tolerance of heavy metal. The study revealed that AMF can regulate the rhizosphere bacterial and fungal composition and foster certain beneficial microbes to enhance the tolerance of maize under La stress. Phytoremediation assisted by AMF is an attractive approach to ameliorate REEs-contaminated soils.