Transcriptional analysis of nitrogen fixation in Paenibacillus durus during growth in nitrogen‐enriched medium,Letters in Applied Microbiology

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Transcriptional analysis of nitrogen fixation in Paenibacillus durus during growth in nitrogen‐enriched medium
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-02-01 , DOI: 10.1111/lam.13455
Mardani Abdul Halim _{1,

2} , Quok‐Cheong Choo ₃ , Hamzah Ahmad Ghazali Amir ₁ , Mustafa Fadzil Farid Wajidi ₄ , Nazalan Najimudin ₁

Affiliation

Paenibacillus durus strain ATCC 35681^T is a Gram‐positive diazotroph that displayed capability of fixing nitrogen even in the presence of nitrate or ammonium. However, the nitrogen fixation activity was detected only at day 1 of growth when cultured in liquid nitrogen‐enriched medium. The transcripts of all the nifH homologues were present throughout the 9‐day study. When grown in nitrogen‐depleted medium, nitrogenase activities occurred from day 1 until day 6 and the nifH transcripts were also present during the course of the study albeit at different levels. In both studies, the absence of nitrogen fixation activity regardless of the presence of the nifH transcripts raised the possibility of a post‐transcriptional or post‐translational regulation of the system. A putative SigA box sequence was found upstream of the transcription start site of nifB1, the first gene in the major nitrogen fixation cluster. The upstream region of nifB2 showed a promoter recognizable by SigE, a sigma factor normally involved in sporulation.

中文翻译：

富氮培养基中生长过程中对芽孢杆菌的固氮作用的转录分析

钝芽孢杆菌ATCC 35681 ^T是革兰氏阳性重氮营养菌，即使在存在硝酸盐或铵盐的情况下，也具有固定氮的能力。然而，当在富含液态氮的培养基中培养时，仅在生长的第1天才检测到固氮活性。在整个9天的研究中，所有nifH同源物的转录本都存在。当在贫氮培养基中生长时，固氮酶活性从第1天到第6天一直存在，并且在研究过程中也存在nifH转录本，尽管其水平不同。在两项研究中，无论是否存在nifH，都没有固氮活性笔录提高了对该系统进行转录后或翻译后调控的可能性。在主要固氮簇中第一个基因nifB1的转录起始位点上游发现了一个推定的SigA盒序列。nifB2的上游区域显示了一个可被SigE识别的启动子，SigE是通常与孢子形成有关的sigma因子。

更新日期：2021-04-12

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