Collagen-Based Thiol–Norbornene Photoclick Bio-Ink with Excellent Bioactivity and Printability,ACS Applied Materials & Interfaces

当前位置： X-MOL 学术 › ACS Appl. Mater. Interfaces › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Collagen-Based Thiol–Norbornene Photoclick Bio-Ink with Excellent Bioactivity and Printability
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2021-01-31 , DOI: 10.1021/acsami.0c16714
Kai Guo _{1,

2} , Heran Wang _{1,

2,

3} , Shijie Li _{1,

2} , Hui Zhang _{1,

2,

3} , Song Li _{1,

2} , Huixuan Zhu _{1,

2,

3} , Zhenda Yang _{1,

2} , Liming Zhang _{1,

2} , Peng Chang ₄ , Xiongfei Zheng _{1,

2}

Affiliation

As the essential foundation of bioprinting technology, cell-laden bio-ink is confronted with the inevitable contradiction between printability and bioactivity. For example, type I collagen has been widely applied for its excellent biocompatibility; however, its relatively low self-assembly speed restricts the performance in high-precision bioprinting of cell-laden structures. In this study, we synthesize norbornene-functionalized neutral soluble collagen (NorCol) by the reaction of acid-soluble collagen (Col) and carbic anhydride in the aqueous phase. NorCol retains collagen triple-helical conformation and can be quickly orthogonally cross-linked to build a cell-laden hydrogel via a cell-friendly thiol-ene photoclick reaction. Moreover, the additional carboxyl groups produced in the reaction of carbic anhydride and collagen obviously improve the solubility of NorCol in neutral buffer and miscibility of NorCol with other polymers such as alginate and gelatin. It enables hybrid bio-ink to respond to multiple stimuli, resulting in continuous cross-linked NorCol networks in hybrid hydrogels. For the first time, the collagen with a triple helix structure and gelatin can be mixed and printed, keeping the integrity of the printed construct after gelatin’s dissolution. The molecular interaction among giant collagen molecules allows NorCol hydrogel formation at a low concentration, which leads to excellent cell spreading, migration, and proliferation. These properties give NorCol flexible formability and excellent biocompatibility in temperature-, ion-, and photo-based bioprinting. We speculate that NorCol is a promising bio-ink for emerging demands in tissue engineering, regenerative medicine, and personalized therapeutics.

中文翻译：

胶原基硫醇-降冰片烯Photoclick生物油墨，具有出色的生物活性和可印刷性

作为生物打印技术的重要基础，充满细胞的生物墨水面临着可印刷性和生物活性之间不可避免的矛盾。例如，I型胶原蛋白因其出色的生物相容性而得到广泛应用。但是，其相对较低的自组装速度限制了充满细胞结构的高精度生物打印性能。在这项研究中，我们通过酸溶性胶原蛋白（Col）和碳酸酐在水相中的反应合成降冰片烯官能化的中性可溶性胶原蛋白（NorCol）。NorCol保留了胶原蛋白的三螺旋构象，并可以通过对细胞友好的硫醇-烯光点击反应快速正交交联，从而形成充满细胞的水凝胶。而且，羧酸酐和胶原蛋白反应中产生的额外羧基明显改善了NorCol在中性缓冲液中的溶解度，以及NorCol与其他聚合物（如藻酸盐和明胶）的混溶性。它使混合生物墨水能够响应多种刺激，从而在混合水凝胶中产生连续的交联NorCol网络。具有三螺旋结构的胶原蛋白和明胶可以首次混合和印刷，从而在明胶溶解后保持印刷结构的完整性。巨大的胶原分子之间的分子相互作用允许低浓度的NorCol水凝胶形成，从而导致出色的细胞扩散，迁移和增殖。这些特性使NorCol在基于温度，离子和照片的生物打印中具有灵活的可成型性和出色的生物相容性。

更新日期：2021-02-17

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南