Three undescribed dammarane-type saponins, russelliinosides A-C, together with a common sterol (β-sitosterol), an abietane diterpenoid (18-hydroxyferruginol), two oleane triterpenoids (daturaolone and oleanolic acid), an ursane triterpenoid (ursolic acid) as well as three 5-hydroxyflavones (cirsimaritin, eupatorin, and salvigenin) were isolated from a dichloromethane extract of the aerial parts of Salvia russellii Benth. The chemical structures of the aforementioned compounds were characterized, using detailed spectroscopic analyses, including high-resolution mass spectrometry and 1D and 2D NMR (¹H–¹H COSY, TOCSY, HSQC, HMBC and NOESY) spectroscopy as well as physicochemical properties. Cytotoxic effects of S. russellii extract and the three isolated russelliinosides were tested against MCF-7 human breast and A549 lung cancer, as well as non-cancer NIH/3T3 cells using MTT reduction assay. Russelliinosides A and B exhibited cytotoxic activities with IC₅₀ values of 7.1 and 30.7 μg/ml against MCF-7 and 33.9 and 69.4 μg/ml against A549 cells, respectively, while russelliinoside C did not show cytotoxicity against cancer cells. On the other hand, russelliinoside A showed an IC₅₀ value of 31.5 μg/ml against NIH/3T3 cells, while russelliinosides B and C had no effect on the viability of these non-cancer cells.

三种未描述的达玛烷型皂苷，罗素苷 AC，以及一种常见的甾醇（β-谷甾醇）、一种松香二萜类化合物（18-羟基铁鲁甘油醇）、两种齐墩果烷三萜类化合物（曼陀罗酮和齐墩果酸）、一种熊果烷三萜类化合物（乌索酸）以及从鼠尾草地上部分的二氯甲烷提取物中分离出三种 5-羟基黄酮（西西玛苷、泽兰素和丹参素）。使用详细的光谱分析（包括高分辨率质谱和 1D 和 2D NMR（ ¹ H- ¹ H COSY、TOCSY、HSQC、HMBC 和 NOESY）光谱以及物理化学性质）对上述化合物的化学结构进行了表征。使用 MTT 还原测定测试了罗氏链球菌提取物和三种分离的罗素苷对 MCF-7 人乳腺癌和 A549 肺癌以及非癌 NIH/3T3 细胞的细胞毒性作用。罗素苷A和B表现出细胞毒性活性，对MCF-7的IC ₅₀值分别为7.1和30.7 μg/ml，对A549细胞的IC 50 值分别为33.9和69.4 μg/ml，而罗素苷C对癌细胞没有表现出细胞毒性。另一方面，罗素素A对NIH/3T3细胞的IC ₅₀值为31.5μg/ml，而罗素素B和C对这些非癌细胞的活力没有影响。