Visualization of the microstructure of hydrogels and the distribution of individual components in a binary polysaccharide hydrogel is often challenging due to its high-water content (up to 99%) and the difficulty in covalent labelling of polysaccharides without important negative effects on the physio-chemical properties of the polysaccharides. The present study investigated the potential use of 5-(4,6-dichlorotriazinyl) aminofluorescein (DTAF) to label polysaccharides that carry different functional groups on the polymer backbone, especially anionic functional groups. Agar, konjac glucomannan (KGM), low acyl gellan, and κ- and ι-carrageenan were covalently labelled with DTAF. The advantage of polysaccharide labelling with DTAF was that the labelling could be done in an aqueous environment. The results suggested that DTAF labelling had no impact on the sol-gel transition temperature and gel strength of polysaccharides. Furthermore, DTAF labelled polysaccharides could be stored as a powder at room temperature for three months, except ι-carrageenan, without losing visualization using CLSM and affecting the mechanical strength. Both hydrated and dehydrated forms of binary polysaccharide composite gels were visualized using CLSM. CLSM images enable visibility of component distribution, compatibility of the two polysaccharides and network formation of both polysaccharides of agar:KGM, agar:potato starch, and KGM:potato starch composite gels.

由于其高水含量（高达99％）以及难以对多糖进行共价标记而又不对物理化学产生重大负面影响，因此水凝胶微观结构的可视化以及二元多糖水凝胶中各个成分的分布通常具有挑战性。多糖的性质。本研究调查了5-（4,6-二氯三嗪基）氨基荧光素（DTAF）标记在聚合物主链上带有不同官能团（尤其是阴离子官能团）的多糖的潜在用途。琼脂，魔芋葡甘露聚糖（KGM），低酰基结冷胶以及κ-和α-角叉菜胶用DTAF共价标记。用DTAF标记多糖的优点是可以在水性环境中进行标记。结果表明DTAF标记对多糖的溶胶-凝胶转变温度和凝胶强度没有影响。此外，DTAF标记的多糖可以以粉末形式在室温下保存除α-角叉菜胶以外的三个月，而不会丢失使用CLSM的可视化和影响机械强度。使用CLSM可视化水合形式和脱水形式的二元多糖复合凝胶。CLSM图像可实现琼脂：KGM，琼脂：马铃薯淀粉和KGM：马铃薯淀粉复合凝胶的组分分布，两种多糖的相容性以及两种多糖的网络形成的可见性。除了α-角叉菜胶以外，不损失使用CLSM的可视化并影响机械强度。使用CLSM可视化水合形式和脱水形式的二元多糖复合凝胶。CLSM图像可实现琼脂：KGM，琼脂：马铃薯淀粉和KGM：马铃薯淀粉复合凝胶的组分分布，两种多糖的相容性以及两种多糖的网络形成的可见性。除了α-角叉菜胶以外，不损失使用CLSM的可视化并影响机械强度。使用CLSM可视化水合形式和脱水形式的二元多糖复合凝胶。CLSM图像可实现琼脂：KGM，琼脂：马铃薯淀粉和KGM：马铃薯淀粉复合凝胶的组分分布，两种多糖的相容性以及两种多糖的网络形成的可见性。