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Expressing Phosphate Transporter PvPht2;1 Enhances P Transport to the Chloroplasts and Increases Arsenic Tolerance in Arabidopsis thaliana
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2021-01-26 , DOI: 10.1021/acs.est.0c03316
Huayuan Feng 1 , Xinyuan Li 1 , Dan Sun 2 , Yanshan Chen 3 , Guohua Xu 4 , Yue Cao 1, 2, 5 , Lena Q. Ma 6
Affiliation  

Arsenic (As) contamination in soils is of great concerns due to its toxicity to plants. As an analogue, phosphorus plays an important role in protecting plants from As toxicity. In this study, we identified a new phosphate transporter 2 (PHT2), PvPht2;1, from As-hyperaccumulator Pteris vittata and analyzed its functions in As and P transport in a yeast mutant, and model plant Arabidopsis thalian. PvPht2;1 contained 12 transmembrane domains, sharing high identity with PHT2 genes in diverse plants. Further, independent of external P or As levels, PvPht2;1 was mainly expressed in P. vittata fronds with the expression being 3–4 folds higher than that in the roots and rhizomes. Localized to the chloroplasts based on GFP-fused PvPht2;1 in model plant tobacco, PvPht2;1 functioned as a low-affinity P transporter. Under As exposure, PvPht2;1 yeast transformants showed comparable growth with the control while high-affinity P transporter PvPht1;3 transformants showed better growth, suggesting that PvPht2;1 transported P but slower than PvPht1;3 transporter. Expressing PvPht2;1 in A. thaliana increased its shoot P concentration without influencing its As accumulation. Further, the chloroplasts’ P content in transgenic A. thaliana increased by 37–59% than wild-type (WT) plants. Under As exposure, the photosynthesis of PvPht2;1-expressing A. thaliana remained stable but that of WT plants decreased. The data indicate that, under As stress, expressing PvPht2;1 in A. thaliana enhanced its P transport to the chloroplasts and protected its photosynthesis. In short, highly expressed in the fronds and not impacted by As exposure, chloroplast-located PvPht2;1 may have protected As-hyperaccumulator P. vittata from As toxicity by efficiently transporting only P to its chloroplasts.

中文翻译:

表达磷酸转运蛋白PvPht2; 1增强了拟南芥中磷向叶绿体的转运并提高了砷的耐受性

由于砷对植物的毒性,土壤中的砷(As)污染备受关注。作为类似物,磷在保护植物免受As毒害方面起着重要作用。在这项研究中,我们从超级蓄积体凤尾蕨中鉴定了一种新的磷酸盐转运蛋白2(PHT2)PvPht2; 1,并分析了其在酵母突变体中的As和P转运中的功能,并模拟了拟南芥拟南芥。PvPht2; 1包含12个跨膜结构域,与多种植物中的PHT2基因具有高度同一性。此外,与外部P或As水平无关,PvPht2; 1主要表达在P. vittata叶的表达比根和根状茎高3-4倍。PvPht2; 1位于模型植物烟草中基于GFP融合的PvPht2; 1的叶绿体中,起低亲和力P转运蛋白的作用。在As暴露下,PvPht2; 1酵母转化体显示出与对照相当的生长,而高亲和力P转运蛋白PvPht1; 3转化体显示出更好的生长,表明PvPht2; 1转运P但比PvPht1; 3转运体慢。在拟南芥中表达PvPht2; 1可增加其茎中P的浓度,而不会影响其As的积累。此外,转基因拟南芥中叶绿体的P含量比野生型(WT)植物增加了37–59%。在砷暴露下,PvPht2; 1的光合作用拟南芥表达稳定,但野生植物下降。这些数据表明,由于应力作用下,表达PvPht2; 1拟南芥增强其p运输到叶绿体和保护它的光合作用。简而言之,叶绿体中的PvPht2; 1在叶中高度表达且不受As暴露的影响,它可能通过仅将P高效转运到叶绿体上而保护了As- hyperaccumulator P. vittata免受As毒性。
更新日期:2021-02-16
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