Sporopollenin Exine微胶囊作为益生菌的潜在肠道递送系统,Small

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Sporopollenin Exine微胶囊作为益生菌的潜在肠道递送系统
Small ( IF 13.0 ) Pub Date : 2021-01-27 , DOI: 10.1002/smll.202004573
Konstantinos Stamatopoulos ₁ , Vasiliki Kafourou ₁ , Hannah K Batchelor ₂ , Spyros J Konteles ₃

Affiliation

尽管对细菌的封装进行了数十年的研究，但是大多数提议的技术都是固定化培养物的形式。在这项工作中，首次描述了孢粉花粉外膜胶囊（SEC）的打开，它使用充当压制微探针的二氧化硅颗粒，并装有干酪乳杆菌（L. casei）细胞。与直接压缩SEC（0.99％）相比，建议的封装提供了约30倍的封装产量（30.87％）。在体外模拟的代表人胃肠道（GI）的禁食和进料培养基中，封装的干酪乳杆菌细胞的存活率比游离细胞高1.21倍和2.25倍。封装的干酪乳杆菌会在SEC内部扩散，产生足够的压力，使SEC破裂并释放出有活力的和代谢活跃的细胞。与将SEC用作封装手段的明显区别在于，SEC可以充当生物反应器，并为封装的细胞提供时间，使其在SEC爆发后被释放之前成千上万倍。SEC的独特优势以及拟议的封装方法，证明了SEC作为益生菌向人胃肠道远端传递系统的潜在应用。

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Sporopollenin Exine Microcapsules as Potential Intestinal Delivery System of Probiotics

Despite several decades of research into encapsulation of bacteria, most of the proposed technologies are in the form of immobilized cultures. In this work, sporopollenin exine capsules (SECs) opened, using silica particles which act as pressing micro‐probes, and loaded with Lactobacillus casei (L. casei) cells, are described for the first time. The proposed encapsulation provided ≈30× higher encapsulation yield (30.87%), compared to direct compression of SECs (0.99%). Encapsulated L. casei cells show 1.21‐ and 2.25‐folds higher viability compared to free cells, in in vitro simulated fasted and fed media representing the human gastrointestinal (GI) tract, respectively. Encapsulated L. casei can proliferate inside the SECs, generating enough pressure to cause the SECs to burst and release the viable and metabolically active cells. The noticeable difference with the application of the SECs as a means of encapsulation is that the SECs may act as a bioreactor and provide time for the encapsulated cells to multiply thousands of times before being released, following the SEC's burst. The unique advantages of SECs alongside the proposed encapsulation method, demonstrates the potential application of SECs as delivery system of probiotics to the distal part of the human GI tract.

更新日期：2021-02-18

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