Injuries to the nervous system affect more than one billion people worldwide, and dramatically impact on the patient’s quality of life. The present work aimed to design and develop a gellan gum (GG)-based composite system for the local delivery of the neuroprotective sigma-1 receptor agonist, 1-[3-(1,1′-biphen)-4-yl] butylpiperidine (RC-33), as a potential tool for the treatment of tissue nervous injuries. The system, consisting of cross-linked electrospun nanofibers embedded in a RC-33-loaded freeze-dried matrix, was designed to bridge the lesion gap, control drug delivery and enhance axonal regrowth. The gradual matrix degradation should ensure the progressive interaction between the inner fibrous mat and the surrounding cellular environment. Nanofibers, prepared by electrospinning polymeric solutions containing GG, two different grades of poly (ethylene oxide) and poloxamer, were cross-linked with calcium ions. GG-based matrices, loaded with different amounts of RC-33, were prepared by freeze-drying. Dialysis studies and solid-state characterization pointed out the formation of an interaction product between GG and RC-33. RC-33-loaded freeze-dried matrices were characterized by the capability to absorb a high buffer content, forming a gel with marked viscoelastic properties, and by RC-33 controlled release properties. The presence of cross-linked nanofibers increased matrix mechanical resistance.

中文翻译：

---

基于结冷胶的复合系统作为治疗神经组织损伤的潜在工具：交联的电纺纳米纤维嵌入RC-33负载的冻干基质中

神经系统损伤影响全球超过十亿人，并极大地影响患者的生活质量。本工作旨在设计和开发基于吉兰糖胶（GG）的复合系统，用于局部递送神经保护性sigma-1受体激动剂1- [3-（1,1'-biphen）-4-基]丁基哌啶（RC-33），作为治疗组织神经损伤的潜在工具。该系统由嵌入RC-33负载的冻干基质中的交联电纺纳米纤维组成，旨在弥合病变间隙，控制药物输送并增强轴突再生。逐渐的基质降解应确保内部纤维垫与周围细胞环境之间的相互作用。通过静电纺丝含GG的聚合物溶液制备的纳米纤维，两种不同等级的聚环氧乙烷和泊洛沙姆与钙离子交联。通过冷冻干燥制备装载有不同量的RC-33的基于GG的基质。透析研究和固态表征指出了GG与RC-33之间相互作用产物的形成。负载RC-33的冻干基质的特征是能够吸收高缓冲液，形成具有显着粘弹性的凝胶以及RC-33控释特性。交联纳米纤维的存在增加了基体的机械阻力。透析研究和固态表征指出了GG与RC-33之间相互作用产物的形成。负载RC-33的冻干基质的特征是能够吸收高缓冲液，形成具有显着粘弹性的凝胶以及RC-33控释特性。交联纳米纤维的存在增加了基体的机械阻力。透析研究和固态表征指出了GG和RC-33之间相互作用产物的形成。负载RC-33的冻干基质的特征是能够吸收高缓冲液，形成具有显着粘弹性的凝胶以及RC-33控释特性。交联纳米纤维的存在增加了基体的机械阻力。