Clomazone, a widely used herbicide, is mainly used in soybean fields. We previously found that clomazone alters Proteobacteria and Nitrospirae abundances and also alters urease activity, which result in changes in NH₄⁺ and NO₃⁻ contents in soil nitrogen cycling. It remains unknown, however, how the co-occurrence patterns of species and functions of soil ecosystems change in response to clomazone applications in soil. We designed a 3-month greenhouse experiment to investigate soil microorganism dynamics in response to clomazone. Clomazone was applied at three doses (e.g., T1, T10, T100), which significantly increased bacterial abundance at days 15 and 60. Fungal abundance was stimulated at day 30 in T10-treated soils, whereas fungal abundances decreased in T100-treated soils at day 15. Clomazone altered bacterial and fungal community structures. Network analyses showed more complex and highly connected microbial communities in clomazone-treated soils. Moreover, an Acidobacteria-dominated cluster was identified within each network of clomazone-treated soils. Clomazone applied at the recommended rate decreased the functional groups that were associated with denitrification and hydrogen oxidation at days 15 and 60, and enhanced photoheterotrophy from days 30 to 60. High clomazone inputs increased trophic types (e.g., chemoheterotrophy, phototrophy, photoautotrophy and cyanobacteria) and C cycling functional groups (e.g., fermentation and cellulolysis). The half-life of clomazone ranged from 40.1 to 93.5 days in three cases. Our results provide important information for use of this herbicide.

广灭灵是一种广泛使用的除草剂，主要用于大豆田。我们先前发现，广灭灵可改变变形杆菌和硝化螺旋菌的丰度，也可改变脲酶的活性，从而导致NH ₄ ⁺和NO _3-的变化^。氮素循环中的含量 然而，仍然未知土壤中生态系统的物种和功能的共现模式如何响应于土壤中的广灭灵施用。我们设计了一个为期3个月的温室实验，以研究土壤微生物对氯满宗的响应。在3个剂量（例如T1，T10，T100）上施用了广灭灵，这在第15天和第60天显着增加了细菌的丰度。在T10处理的土壤中，真菌丰度在第30天被刺激，而在T100处理的土壤中，真菌丰度在30天时降低了。第15天。灭草zone改变了细菌和真菌的群落结构。网络分析显示，在广灭灵处理过的土壤中存在更复杂和高度相关的微生物群落。此外，酸性细菌在每个广灭灵处理过的土壤网络中均发现了主要的簇。在第15天和第60天，以推荐的比例使用的广灭灵减少了与反硝化和氢氧化有关的官能团，而从第30天到60天则增强了光异养作用。高的广灭草投入物增加了营养类型（例如，趋化性，光养性，光自养性和蓝细菌）。和C循环官能团（例如，发酵和纤维素分解）。在三例中，广灭灵的半衰期为40.1至93.5天。我们的结果为该除草剂的使用提供了重要信息。