The low sensitivity of fluorescent sensors limits the on-site and visual detection of seafood freshness. To address these issues, a novel intelligent nanofiber ratiometric fluorescent sensor, known as a BAsCNF, was designed using cellulose nanofibers (CNFs) produced by TEMPO oxidation (TO-CNFs) as the skeleton, and fluorescein isothiocyanate and proporphyrin IX modified nanofibers as the indicator and internal reference, respectively. TO-CNFs with nano-scale characteristics improved the air permeability of BAsCNF films, which endowed the sensor with excellent response performance to biogenic amines (BAs). Thus, the BAsCNF produced obvious colour changes from red to yellow-green in response to an increased concentration of BAs, where the limit of detection for BAs was as low as 1 ppm. Meanwhile, the discoloration trend of the BAsCNF in the freshness detection of shrimp and pork was consistent with the variation of the key indicators during storage, such as the content of total volatile basic nitrogen and the total number of colonies. The results suggest that the BAsCNF sensor possessed high detection accuracy and broad application prospects in the field of food safety.

荧光传感器的低灵敏度限制了海鲜新鲜度的现场和视觉检测。为了解决这些问题，设计了一种新型的智能纳米纤维比率荧光传感器，称为BAsCNF，使用通过TEMPO氧化（TO-CNF）生产的纤维素纳米纤维（CNF）作为骨架，并使用异硫氰酸荧光素和原卟啉IX修饰的纳米纤维作为指示剂。和内部参考。具有纳米级特性的TO-CNF改善了BAsCNF薄膜的透气性，这赋予了传感器对生物胺（BAs）的出色响应性能。因此，随着BAs浓度的增加，BAsCNF产生了从红色到黄绿色的明显颜色变化，其中BAs的检出限低至1 ppm。与此同时，虾和猪肉新鲜度检测中BAsCNF的褪色趋势与储存过程中关键指标（如总挥发性碱性氮含量和菌落总数）的变化一致。结果表明，BAsCNF传感器具有很高的检测精度，在食品安全领域具有广阔的应用前景。