Alicyclobacillus acidoterrestris is the main hazardous factor leading to deterioration of commercialized fruit juices, as it can result in serious economic loss to the industry. Currently detection methods require high-end equipment with low specificity and sensitivity. To address these problems, we describe herein a monoclonal antibody with high specificity to A. acidoterrestris using a cell wall protein as antigen. A colorimetric and fluorescence dual-mode immunoassay system employing the prepared antibody was then developed based on the quenching effect of oxidized p-phenylenediamine on fluorescein. This approach could accurately differentiate between the target of interest and other bacteria species with enhanced limit of detection of 4.8 × 102 CFU/mL. Apple juice artificially contaminated with A. acidoterrestris at the concentration of 1 CFU/mL could be determined as positive after 24 h enrichment. The resulting immunoassay platform provided a rapid, convenient, sensitive, and selective way for detecting A. acidoterrestris, holding great potential as a robust tool for preventing spoilage in the fruit juice industry.

酸热脂环酸杆菌是导致商业化果汁恶化的主要危险因素，因为它可能给行业造成严重的经济损失。当前的检测方法需要具有低特异性和灵敏度的高端设备。为了解决这些问题，我们在本文中描述了一种使用细胞壁蛋白作为抗原对嗜酸曲霉具有高度特异性的单克隆抗体。然后，基于氧化的p的猝灭作用，开发了采用所制备抗体的比色和荧光双模式免疫分析系统。-苯二胺在荧光素上。这种方法可以准确区分目标和其他细菌，检测限提高到4.8×102 CFU / mL。富集24小时后，浓度为1 CFU / mL的被酸曲霉人工污染的苹果汁可以确定为阳性。所得的免疫测定平台提供了一种快速，方便，灵敏和选择性的方法来检测酸曲霉，其作为防止果汁工业变质的强大工具具有巨大潜力。