TiO2 纳米颗粒的短预辐照增加了对人肺共培养系统的细胞毒性,Chemical Research in Toxicology

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TiO2 纳米颗粒的短预辐照增加了对人肺共培养系统的细胞毒性
Chemical Research in Toxicology ( IF 3.7 ) Pub Date : 2021-01-17 , DOI: 10.1021/acs.chemrestox.0c00354
Ozge Kose ₁ , Maura Tomatis ₂ , Francesco Turci ₂ , Naila-Besma Belblidia _{3,

4} , Jean-François Hochepied _{3,

4} , Jérémie Pourchez ₁ , Valérie Forest ₁

Affiliation

锐钛矿二氧化钛纳米粒子 (TiO ₂ NPs) 主要由于其光催化性能而被广泛用于工业应用。在进入肺部之前，几乎所有 TiO ₂ NPs 都暴露在一些紫外线下，并且 TiO ₂ NPs 的肺毒性可能会受到光激发的影响，光激发会改变 TiO ₂表面特性。尽管已经对 TiO ₂ NPs 的毒性进行了广泛的研究，但关于预先暴露于紫外线的 TiO ₂ NPs的毒性数据有限，它们对人类的影响仍然未知。在本研究中，五种类型的 TiO ₂具有定制物理化学特征的纳米颗粒被表征并用紫外线照射 30 分钟。辐射后，评估了人肺共培养系统（A549 上皮细胞和巨噬细胞从 THP-1 细胞分化而来）的细胞毒性、促炎反应和氧化应激。紫外光照射TiO ₂ NPs后，所有样品的表面电荷负性较小，平均聚集体尺寸略有增加。与未辐照的样品相比，预辐照的 TiO ₂ NPs具有更高的细胞毒性作用。TiO ₂ NPs 的预辐照对促炎反应和氧化应激没有显着影响，如 IL-8、TNF-α 和活性氧的类似产生所示。

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Short Preirradiation of TiO2 Nanoparticles Increases Cytotoxicity on Human Lung Coculture System

Anatase titanium dioxide nanoparticles (TiO₂ NPs) are used in a large range of industrial applications mainly due to their photocatalytic properties. Before entering the lung, virtually all TiO₂ NPs are exposed to some UV light, and lung toxicity of TiO₂ NPs might be influenced by photoexcitation that is known to alter TiO₂ surface properties. Although the TiO₂ NPs toxicity has been extensively investigated, limited data are available regarding the toxicity of TiO₂ NPs that have been pre-exposed to UV light, and their impact on humans remains unknown. In this study, five types of TiO₂NPs with tailored physicochemical features were characterized and irradiated by UV for 30 min. Following irradiation, cytotoxicity, pro-inflammatory response, and oxidative stress on a human lung coculture system (A549 epithelial cells and macrophages differentiated from THP-1 cells) were assessed. The surface charge of all samples was less negative after UV irradiation of TiO₂ NPs, and the average aggregate size was slightly increased. A higher cytotoxic effect was observed for preirradiated TiO₂ NPs compared to nonirradiated samples. Preirradiation of TiO₂ NPs had no significant impact on the pro-inflammatory response and oxidative stress as shown by a similar production of IL-8, TNF-α, and reactive oxygen species.

更新日期：2021-03-15

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