Oroxin B, a flavonoid, is a major bioactive component form Oroxylum indicum (L.) Vent. with enormous anti-hepatoma effects. To data, the oroxin B metabolism studies remain underexplored. This study was designed to characterize oroxin B metabolism in vivo and in vitro by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS). Consequently, 30 metabolites in rats, 8 metabolites in liver microsomes and 18 metabolites in intestinal bacteria were identified, and 9 metabolites were recognized by comparison with standards. The biotransformation processes involved ketone, acetylation, loss of C₁₂H₂₀O₁₀, and loss of C₆H₁₀O₅. And baicalein and oroxin A were generated after loss of C₁₂H₂₀O₁₀, and loss of C₆H₁₀O₅, respectively, and further went through some other reactions, such as oxidation, methylation, internal hydrolysis, hydrogenation, loss of O, ketone, glycine conjugation, glucuronide conjugation and their composite reactions. The results provide valuable evidence for elucidation the potential mechanism of oroxin B pharmacological action, and offer reasonable guidelines for further investigations of oroxin B safety and efficacy.

Oroxin B（一种类黄酮）是Oroxylum indicum（L.）Vent的主要生物活性成分。具有巨大的抗肝癌作用。据数据，oroxin B代谢研究仍未得到充分开发。本研究旨在通过超高效液相色谱结合四极杆飞行时间质谱（UHPLC-Q-TOF-MS / MS）表征体内和体外的oroxin B代谢。结果，鉴定出大鼠中的30种代谢物，肝微粒体中的8种代谢物和肠细菌中的18种代谢物，并通过与标准品的比较识别出9种代谢物。生物转化过程涉及酮，乙酰化，C ₁₂ H ₂₀ O _10的损失和C的损失₆ H ₁₀ O ₅。黄e素和Oroxin A分别在失去C ₁₂ H ₂₀ O ₁₀和失去C ₆ H ₁₀ O ₅后产生，并进一步经历了一些其他反应，如氧化，甲基化，内部水解，氢化，失活。 O，酮，甘氨酸结合，葡糖苷酸结合及其复合反应。该结果为阐明牛毒素B药理作用的潜在机制提供了有价值的证据，并为进一步研究牛毒素B的安全性和有效性提供了合理的指导。