Mitochondrial dysregulation controls cell death and survival by changing endogenous molecule concentrations and ion flows across the membrane. Here, we report the design of a triply emissive nanoscale metal-organic layer (nMOL), NA@Zr-BTB/F/R, for sensing mitochondrial dysregulation. Zr-BTB nMOL containing Zr6 secondary building units (SBUs) and 2,4,6-tris(4-carboxyphenyl)aniline (BTB-NH2) ligands was postsynthetically functionalized to afford NA@Zr-BTB/F/R by exchanging formate capping groups on the SBUs with glutathione(GSH)-selective (2E)-1-(2'-naphthyl)-3-(4-carboxyphenyl)-2-propen-1-one (NA) and covalent conjugation of pH-sensitive fluorescein (F) and GSH/pH-independent rhodamine-B (R) to the BTB-NH2 ligands. Cell imaging demonstrated NA@Zr-BTB/F/R as a ratiometric sensor for mitochondrial dysregulation and chemotherapy resistance via GSH and pH sensing.

中文翻译：

---

纳米级金属-有机层通过谷胱甘肽和 pH 的比率传感检测线粒体失调和化学抗性

线粒体失调通过改变内源性分子浓度和跨膜离子流来控制细胞死亡和存活。在这里，我们报告了三重发射纳米级金属有机层 (nMOL) NA@Zr-BTB/F/R 的设计，用于感测线粒体失调。含有 Zr6 二级构建单元 (SBU) 和 2,4,6-三(4-羧基苯基)苯胺 (BTB-NH2) 配体的 Zr-BTB nMOL 经合成后功能化，通过交换甲酸封端得到 NA@Zr-BTB/F/R SBU 上的基团与谷胱甘肽 (GSH)-选择性 (2E)-1-(2'-萘基)-3-(4-羧基苯基)-2-propen-1-one (NA) 和 pH 敏感荧光素的共价结合(F) 和 GSH/pH 无关的罗丹明-B (R) 到 BTB-NH2 配体。