自然启发的多酶固定化：策略和概念,ACS Applied Bio Materials

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自然启发的多酶固定化：策略和概念
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2021-01-14 , DOI: 10.1021/acsabm.0c01293
Nidhi C Dubey ₁ , Bijay P Tripathi ₂

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在生物系统中，密集细胞环境中酶的时空排列、亚细胞区室、细胞表面上的膜相关酶复合物、支架组织的蛋白质、蛋白质簇和模块化酶为可能的多酶固定化设计提供了许多范式。人工适应。在代谢通道中，参与酶的催化位点足够接近以引导瞬时化合物，从而产生高局部浓度的代谢物，并最大限度地减少竞争途径对同一前体的干扰。多年来，这些现象促使研究人员通过产生多酶融合、通过亲和结构域-配体结合形成簇、交联、蛋白质和核酸的生物分子支架上/中的缀合，以及两亲分子的自组装。这篇综述首先讨论了底物通道策略以及最近综合构建它的经验努力。之后，详细讨论了与增强固定化酶催化性能相关的流行概念。此外，综述的核心部分总结了过去十年自然驱动的多酶组装的进展。在本节中，通过将受自然启发的策略分为三个主要类别来给予特别关注：（i）多酶/结构域复合物模拟（无支架），（ii）固定在生物分子支架上，以及（iii）区室化。特别是，详细的概述与该领域取得的进展的自然对应物相关联。然后，我们讨论了多酶共组装的有益说明，并提供了合理共固定化设计中基本参数的概要。

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Nature Inspired Multienzyme Immobilization: Strategies and Concepts

In a biological system, the spatiotemporal arrangement of enzymes in a dense cellular milieu, subcellular compartments, membrane-associated enzyme complexes on cell surfaces, scaffold-organized proteins, protein clusters, and modular enzymes have presented many paradigms for possible multienzyme immobilization designs that were adapted artificially. In metabolic channeling, the catalytic sites of participating enzymes are close enough to channelize the transient compound, creating a high local concentration of the metabolite and minimizing the interference of a competing pathway for the same precursor. Over the years, these phenomena had motivated researchers to make their immobilization approach naturally realistic by generating multienzyme fusion, cluster formation via affinity domain–ligand binding, cross-linking, conjugation on/in the biomolecular scaffold of the protein and nucleic acids, and self-assembly of amphiphilic molecules. This review begins with the discussion of substrate channeling strategies and recent empirical efforts to build it synthetically. After that, an elaborate discussion covering prevalent concepts related to the enhancement of immobilized enzymes’ catalytic performance is presented. Further, the central part of the review summarizes the progress in nature motivated multienzyme assembly over the past decade. In this section, special attention has been rendered by classifying the nature-inspired strategies into three main categories: (i) multienzyme/domain complex mimic (scaffold-free), (ii) immobilization on the biomolecular scaffold, and (iii) compartmentalization. In particular, a detailed overview is correlated to the natural counterpart with advances made in the field. We have then discussed the beneficial account of coassembly of multienzymes and provided a synopsis of the essential parameters in the rational coimmobilization design.

更新日期：2021-02-15

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