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Autonomous operation of 3D DNA walkers in living cells for microRNA imaging
Nanoscale ( IF 5.8 ) Pub Date : 2020-12-21 , DOI: 10.1039/d0nr06651f
Hui Hu 1, 2, 3, 4 , Fu Zhou 1, 2, 3, 4 , Baojuan Wang 2, 3, 5, 6, 7 , Xin Chang 2, 3, 5, 6, 7 , Tianyue Dai 1, 2, 3, 4 , Ruifen Tian 1, 2, 3, 4 , Yifei Wan 1, 2, 3, 4 , Xiayan Wang 7, 8, 9, 10 , Guangfeng Wang 1, 2, 3, 4
Nanoscale ( IF 5.8 ) Pub Date : 2020-12-21 , DOI: 10.1039/d0nr06651f
Hui Hu 1, 2, 3, 4 , Fu Zhou 1, 2, 3, 4 , Baojuan Wang 2, 3, 5, 6, 7 , Xin Chang 2, 3, 5, 6, 7 , Tianyue Dai 1, 2, 3, 4 , Ruifen Tian 1, 2, 3, 4 , Yifei Wan 1, 2, 3, 4 , Xiayan Wang 7, 8, 9, 10 , Guangfeng Wang 1, 2, 3, 4
Affiliation
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Three dimensional (3D) DNA walkers hold great potential in serving as an ideal candidate for signal transduction and amplification in bio-assays. However, the autonomous operation of 3D DNA walkers inside living cells is still few and far between, which could be attributed to the lack of suitable driving forces and moderate efficiency in terms of the cellular uptake of such complex 3D DNA components. Herein, a newly updated autonomously operated and highly integrated 3D DNA walker on Au nanoparticles (Au NPs)/zeolitic imidazolate framework-8 (ZIF-8) was activated in a tumor microenviroment and its signal amplified assay capability in living cells was demonstrated using miRNA as a sensing model biomolecule. Specifically, we assembled a 3D DNA motor, including Zn2+-dependent DNAzyme and substrates on the AuNPs grafted on ZIF-8. After being delivered into a living cell, ZIF-8 was efficiently degraded in the tumor microenvironment (low pH value), locally releasing the Zn2+ and DNA motor. Then, a self-sufficient DNA motor autonomously performed the bio-analytical task of imaging miRNA-10b, with a low detection limit of 34 pM. Also, such self-sufficient 3D walkers allowed real-time imaging of MDA-MB-231 cells by intracellular operation. This method demonstrates the self-sufficient 3D DNA motor's bioanalytical application in living cells which may inspire various other biological applications including gene delivery, therapy, etc.
中文翻译：
活细胞中3D DNA Walker的自主操作以进行microRNA成像
三维（3D）DNA Walker具有巨大的潜力，可以用作生物测定中信号转导和扩增的理想候选者。但是，活细胞内部3D DNA Walker的自主操作仍然很少，而且可能相距甚远，这可以归因于缺乏合适的驱动力和在细胞摄取此类复杂3D DNA成分方面中等的效率。在本文中，在肿瘤微环境中激活了在Au纳米颗粒（Au NPs）/沸石咪唑盐骨架8（ZIF-8）上新更新的自主操作且高度集成的3D DNA Walker，并使用miRNA证明了其在活细胞中的信号放大测定能力作为传感模型生物分子。具体来说，我们组装了3D DNA电机，包括Zn 2+ZIF-8上嫁接的AuNPs上的非依赖性DNA酶和底物。进入活细胞后，ZIF-8在肿瘤微环境中有效降解（低pH值），局部释放Zn 2+和DNA马达。然后，一个自给自足的DNA马达以34 pM的低检测限自主执行miRNA-10b成像的生物分析任务。同样，这种自给自足的3D Walker可以通过细胞内操作对MDA-MB-231细胞进行实时成像。该方法证明了自足的3D DNA马达在活细胞中的生物分析应用，这可能会激发各种其他生物学应用，包括基因传递，治疗等。
更新日期：2021-01-13
中文翻译：
活细胞中3D DNA Walker的自主操作以进行microRNA成像
三维（3D）DNA Walker具有巨大的潜力，可以用作生物测定中信号转导和扩增的理想候选者。但是，活细胞内部3D DNA Walker的自主操作仍然很少，而且可能相距甚远，这可以归因于缺乏合适的驱动力和在细胞摄取此类复杂3D DNA成分方面中等的效率。在本文中，在肿瘤微环境中激活了在Au纳米颗粒（Au NPs）/沸石咪唑盐骨架8（ZIF-8）上新更新的自主操作且高度集成的3D DNA Walker，并使用miRNA证明了其在活细胞中的信号放大测定能力作为传感模型生物分子。具体来说，我们组装了3D DNA电机，包括Zn 2+ZIF-8上嫁接的AuNPs上的非依赖性DNA酶和底物。进入活细胞后，ZIF-8在肿瘤微环境中有效降解（低pH值），局部释放Zn 2+和DNA马达。然后，一个自给自足的DNA马达以34 pM的低检测限自主执行miRNA-10b成像的生物分析任务。同样，这种自给自足的3D Walker可以通过细胞内操作对MDA-MB-231细胞进行实时成像。该方法证明了自足的3D DNA马达在活细胞中的生物分析应用，这可能会激发各种其他生物学应用，包括基因传递，治疗等。
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