使用超滤-液相色谱-质谱法指导的高速逆流色谱目标筛选和分离黄芩潜在的脂肪氧化酶和超氧化物歧化酶抑制剂,Journal of Separation Science

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使用超滤-液相色谱-质谱法指导的高速逆流色谱目标筛选和分离黄芩潜在的脂肪氧化酶和超氧化物歧化酶抑制剂
Journal of Separation Science ( IF 2.8 ) Pub Date : 2021-01-12 , DOI: 10.1002/jssc.202001072
Jianli Xia ₁ , Chunming Liu ₁ , Huazhou Niu ₁ , Wanchao Hou ₁ , Sainan Li ₁

Affiliation

我们提出了一种基于超滤高效液相色谱结合光电二极管阵列检测器和电喷雾电离质谱的简单有效方法，用于快速筛选和鉴定可从黄芩提取物中获得的配体。五种主要化合物（chrysin-6-C-arabinosyl-8-C-glucoside, chrysin-6-C-glucosyl-8-C-arabinoside, baicalin, oroxylin A-7- O葡萄糖醛酸苷和汉黄芩苷）被鉴定为潜在有效的脂肪氧化酶和超氧化物歧化酶抑制剂。随后，使用乙酸乙酯/乙醇/醋酸水 (0.1%) (1.0:0.1:1.0, v/v/v) 作为溶剂体系，通过高速逆流色谱分离特异性结合配体。据我们所知，这是含有强效脂肪氧化酶和超氧化物歧化酶抑制剂的黄芩提取物的首次报道。我们的结果表明，从黄芩正丁醇层中系统分离出生物活性成分由超滤高效液相色谱法结合光电二极管阵列检测和电喷雾电离质谱法指导，代表了一种可行且有效的技术，也可用于其他酶抑制剂的鉴定和分离。

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Screening and isolation of potential lipoxidase and superoxide dismutase inhibitors from Scutellaria baicalensis Georgi using high‐speed countercurrent chromatography target‐guided by ultrafiltration‐liquid chromatography‐mass spectrometry

We present a simple and efficient method based on ultrafiltration high‐performance liquid chromatography coupled with a photodiode array detector and electrospray ionization mass spectrometry for the rapid screening and identification of ligands obtainable from the extract of Scutellaria baicalensis. Five major compounds (chrysin‐6‐C‐arabinosyl‐8‐C‐glucoside, chrysin‐6‐C‐glucosyl‐8‐C‐arabinoside, baicalin, oroxylin A‐7‐O‐glucuronide, and wogonoside) were identified as potentially effective inhibitors of lipoxidase and superoxide dismutase. Subsequently, specific binding ligands were separated by high‐speed countercurrent chromatography, using ethyl acetate/ethyl alcohol/water acetate (0.1%) (1.0:0.1:1.0, v/v/v) as the solvent system. To the best of our knowledge, this is the first report of S. baicalensis extracts containing potent lipoxidase and superoxide dismutase inhibitors. Our results demonstrate that the systematic isolation of bioactive components from the n‐butyl alcohol layer of S. baicalensis guided by ultrafiltration high‐performance liquid chromatography coupled with photodiode array detection and electrospray ionization mass spectrometry represents a feasible and efficient technique that could also be employed for the identification and isolation of other enzyme inhibitors.

更新日期：2021-01-12

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