<br>分根系统：详细的方法、替代应用以及叶蛋白质组水平的影响,Plant Methods

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分根系统：详细的方法、替代应用以及叶蛋白质组水平的影响
Plant Methods ( IF 4.7 ) Pub Date : 2021-01-09 , DOI: 10.1186/s13007-020-00706-1
Iñigo Saiz-Fernández ₁ , Martin Černý ₂ , Jan Skalák _{2,

3} , Břetislav Brzobohatý _{2,

4,

5}

Affiliation

分根系统（SRS）在植物科学中有许多应用，但根据实验设计，其实施可能很困难且耗时。此外，该系统也不能免受限制，因为建立 SRS 所需的时间限制了植物开发实验的早期进行。在这里，我们优化并详细解释了在拟南芥幼苗中在体外和土壤中建立 SRS 的方法。我们发现，与完全去根相比，部分去根最大限度地缩短了恢复时间，从而允许在较年轻的植物中建立分根系统。对去根过程后拟南芥叶蛋白质组变化的分析强调了完全和部分去根植物在愈合过程中经历的独特代谢变化。该系统还经过了干旱实验的验证，因为它提供了一种将水溶性化合物应用于遭受干旱胁迫的植物的方法。通过在两半缺水的分根系统中种植植物，可以将所需的化合物施用到根系的一半上，一旦化合物被吸收，就可以将其从主植物上切下来，从而最大限度地减少补水并维持干旱条件。部分去根是在拟南芥等小型植物中获得分根系统的建议方法，因为生长参数、存活率和蛋白质组分析表明，与完全去根相比，这是一个压力较小的过程，从而形成最终的莲座区更接近于未切割的植物。此外，我们提供的证据表明，分裂的根系可用于干旱实验，其中施用水溶性化合物时补水的影响最小。

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Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level

Split-root systems (SRS) have many applications in plant sciences, but their implementation, depending on the experimental design, can be difficult and time-consuming. Additionally, the system is not exempt from limitations, since the time required for the establishment of the SRS imposes a limit to how early in plant development experiments can be performed. Here, we optimized and explained in detail a method for establishing a SRS in young Arabidopsis thaliana seedlings, both in vitro and in soil. We found that the partial de-rooting minimized the recovery time compared to total de-rooting, thus allowing the establishment of the split-root system in younger plants. Analysis of changes in the Arabidopsis leaf proteome following the de-rooting procedure highlighted the distinct metabolic alterations that totally and partially de-rooted plants undergo during the healing process. This system was also validated for its use in drought experiments, as it offers a way to apply water-soluble compounds to plants subjected to drought stress. By growing plants in a split-root system with both halves being water-deprived, it is possible to apply the required compound to one half of the root system, which can be cut from the main plant once the compound has been absorbed, thus minimizing rehydration and maintaining drought conditions. Partial de-rooting is the suggested method for obtaining split-root systems in small plants like Arabidopsis thaliana, as growth parameters, survival rate, and proteomic analysis suggest that is a less stressful procedure than total de-rooting, leading to a final rosette area much closer to that of uncut plants. Additionally, we provide evidence that split root-systems can be used in drought experiments where water-soluble compounds are applied with minimal effects of rehydration.

更新日期：2021-01-10

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