Abstract

Investigation of the entomogenous fungus Setosphaeria rostrate LGWB-10 from Harmonia axyridis led to the isolation of four new isocoumarin derivatives, setosphlides A–D (1–4), and four known analogues (5–8). Their planar structures and the relative configurations were elucidated by comprehensive spectroscopic methods. The absolute configurations of isocoumarin nucleus for 1–4 were elucidated by their ECD spectra. The C-10 relative configurations for the pair of C-10 epimers (1 and 2) were established by comparing the magnitude of the computed ¹³C NMR chemical shifts (Δδ_calcd.) with the experimental ¹³C NMR values (Δδ_exp.) for the epimers. All of the isolated compounds (1–8) were evaluated for their cytotoxicities against four human tumor cell lines MCF-7, MGC-803, HeLa, and Huh-7.

Graphic Abstract

中文翻译：

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Setosphlides A–D，来自虫生真菌Setosphaeria的新异香豆素衍生物LGWB-10

摘要

的虫生真菌的调查玉米大斑喙LGWB-10从异色导致四个新的异香豆素衍生物的分离，setosphlides A-d（1 - 4），和四个已知类似物（5 - 8）。通过全面的光谱方法阐明了它们的平面结构和相对构型。为异香豆素核的绝对构型1 - 4被其ECD光谱阐明。通过比较计算出的^13个C-10差向异构体（1和2）的C-10相对构型C NMR化学位移（Δ δ_计算值）与实验^13个C NMR值（Δ δ_浓淡的差向异构体）。所有的分离的化合物（的1 - 8）它们对四种人类肿瘤细胞系MCF-7，MGC-803，HeLa和的Huh-7细胞毒性进行了评价。

图形摘要