基底外侧杏仁核(BLA)和杏仁核中枢核(CEA)已被证明在控制焦虑,压力和恐惧相关行为中起着关键作用。BLA是由γ-氨基丁酸能(GABA能)中间神经元和谷氨酸能神经元组成的皮质样复合物。CEA是杏仁核的纹状样输出,几乎仅由GABA能中突棘神经元组成。在这项研究中,我们使用条件顺行轴突追踪,免疫组织化学和VGAT-Cre转基因小鼠探索了BLA和CEA中GABA能神经元的形态和轴突预测,以进一步了解它们的功能。我们发现,来自BLA的GABA能神经元的轴突投影主要分布于前脑,而来自CEA的GABA能神经元则分布于前脑,中脑和脑干。在前脑中,来自BLA的GABA能神经元的轴突投射到嗅觉前核,大脑皮层,隔膜,纹状体,丘脑,杏仁核和海马体。来自CEA的GABA能神经元的轴突投影分布在前额叶皮层的核,终末皮层的床核,下丘脑和丘脑。在中脑和脑干中,在水管周围的灰质,黑质和蓝斑中发现了来自CEA的GABA能神经元的轴突投影。这些数据揭示了探索BABA和CEA中GABA能神经元功能的神经解剖学基础,尤其是在恐惧相关行为的处理过程中。来自BLA的GABA能神经元的轴突投射到嗅觉前核,大脑皮层,隔膜,纹状体,丘脑,杏仁核和海马体。来自CEA的GABA能神经元的轴突投影分布在前额叶皮层的核,终末皮层的床核,下丘脑和丘脑。在中脑和脑干中,在水管周围的灰质,黑质和蓝斑中发现了来自CEA的GABA能神经元的轴突投影。这些数据揭示了探索BABA和CEA中GABA能神经元功能的神经解剖学基础,尤其是在恐惧相关行为的处理过程中。来自BLA的GABA能神经元的轴突投射到嗅觉前核,大脑皮层,隔膜,纹状体,丘脑,杏仁核和海马体。来自CEA的GABA能神经元的轴突投影分布在前额叶皮层的核,终末皮层的床核,下丘脑和丘脑。在中脑和脑干中,在水管周围的灰质,黑质和蓝斑中发现了来自CEA的GABA能神经元的轴突投影。这些数据揭示了探索BABA和CEA中GABA能神经元功能的神经解剖学基础,尤其是在恐惧相关行为的处理过程中。来自CEA的GABA能神经元的轴突投影分布在前额叶皮层的核,终末皮层的床核,下丘脑和丘脑。在中脑和脑干中,在水管周围的灰质,黑质和蓝斑中发现了来自CEA的GABA能神经元的轴突投影。这些数据揭示了探索BABA和CEA中GABA能神经元功能的神经解剖学基础,尤其是在恐惧相关行为的处理过程中。来自CEA的GABA能神经元的轴突投影分布在前额叶皮层的核,终末皮层的床核,下丘脑和丘脑。在中脑和脑干中,在水管周围的灰质,黑质和蓝斑中发现了来自CEA的GABA能神经元的轴突投影。这些数据揭示了探索BABA和CEA中GABA能神经元功能的神经解剖学基础,尤其是在恐惧相关行为的处理过程中。和蓝斑。这些数据揭示了探索BABA和CEA中GABA能神经元功能的神经解剖学基础,尤其是在恐惧相关行为的处理过程中。和蓝斑。这些数据揭示了探索BABA和CEA中GABA能神经元功能的神经解剖学基础,尤其是在恐惧相关行为的处理过程中。
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Differential efferent projections of GABAergic Neurons in the Basolateral and Central Nucleus of Amygdala in mice
The Basolateral amygdala (BLA) and central nucleus of the amygdala (CEA) have been proved to play a key role in the control of anxiety, stress and fear-related behaviors. BLA is a cortex-like complex consisting of both γ-aminobutyric acidergic (GABAergic) interneurons and glutamatergic neurons. The CEA is a striatum-like output of the amygdala, consisting almost exclusively of GABAergic medium spiny neurons. In this study, we explored the morphology and axonal projections of the GABAergic neurons in BLA and CEA, using conditional anterograde axonal tracing, immunohistochemistry, and VGAT-Cre transgenic mice to further understand their functional roles. We found that the axonal projections of GABAergic neurons from the BLA mainly distributed to the forebrain, whilst GABAergic neurons from the CEA distributed to the forebrain, midbrain and brainstem. In the forebrain, the axonal projections of GABAergic neurons from the BLA projected to the anterior olfactory nucleus, the cerebral cortex, the septum, the striatum, the thalamus, the amygdala and the hippocampus. The axonal projections of GABAergic neurons from the CEA distributed to the nuclei of the prefrontal cortex, the bed nucleus of the stria terminalis, the hypothalamus and the thalamus. In the midbrain and brainstem, the axonal projections of GABAergic neurons from the CEA were found in the periaqueductal gray, the substantia nigra, and the locus coeruleus. These data reveal the neuroanatomical basis for exploring the function of GABAergic neurons in the BLA and CEA, particularly during the processing of fear-related behavior.