DNA Nanoswitch Barcodes for Multiplexed Biomarker Profiling,Nano Letters

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DNA Nanoswitch Barcodes for Multiplexed Biomarker Profiling
Nano Letters ( IF 9.6 ) Pub Date : 2021-01-04 , DOI: 10.1021/acs.nanolett.0c03929
Arun Richard Chandrasekaran ₁ , Molly MacIsaac _{2,

3,

4} , Javier Vilcapoma ₁ , Clinton H Hansen _{2,

3,

4} , Darren Yang _{2,

3,

4} , Wesley P Wong _{2,

3,

4} , Ken Halvorsen ₁

Affiliation

Molecular biomarkers play a key role in the clinic, aiding in diagnostics and prognostics, and in the research laboratory, contributing to our basic understanding of diseases. Detecting multiple and diverse molecular biomarkers within a single accessible assay would have great utility, providing a more comprehensive picture for clinical evaluation and research, but is a challenge with standard methods. Here, we report programmable DNA nanoswitches for multiplexed detection of up to 6 biomarkers at once with each combination of biomarkers producing a unique barcode signature among 64 possibilities. As a defining feature of our method, we show “mixed multiplexing” for simultaneous barcoded detection of different types of biomolecules, for example, DNA, RNA, antibody, and protein in a single assay. To demonstrate clinical potential, we show multiplexed detection of a prostate cancer biomarker panel in serum that includes two microRNA sequences and prostate specific antigen.

中文翻译：

用于多重生物标志物分析的 DNA 纳米开关条形码

分子生物标志物在临床中发挥着关键作用，有助于诊断和预后，在研究实验室中，有助于我们对疾病的基本了解。在单一可访问的测定中检测多种多样的分子生物标志物将具有很大的实用性，为临床评估和研究提供更全面的画面，但对标准方法来说是一个挑战。在这里，我们报告了可编程 DNA 纳米开关，用于同时检测多达 6 种生物标志物，每种生物标志物组合在 64 种可能性中产生独特的条形码签名。作为我们方法的一个定义特征，我们展示了“混合多路复用”，用于在一次检测中同时对不同类型的生物分子进行条形码检测，例如 DNA、RNA、抗体和蛋白质。为了展示临床潜力，

更新日期：2021-01-13

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