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Temperature and pH Dual Responsive Nanogels of Modified Sodium Alginate and NIPAM for Berberine Loading and Release
ACS Omega ( IF 3.7 ) Pub Date : 2021-01-04 , DOI: 10.1021/acsomega.0c03965 Shuhua Chang 1 , Dawei Qin 1 , Rongjun Yan 2 , Mengli Zhang 3 , Binglin Sui 1 , Hui Xu 3 , Zhaomin Zheng 3 , Xiaoli Hou 3 , Yonggang Wang 4 , Chenggang Qi 4
ACS Omega ( IF 3.7 ) Pub Date : 2021-01-04 , DOI: 10.1021/acsomega.0c03965 Shuhua Chang 1 , Dawei Qin 1 , Rongjun Yan 2 , Mengli Zhang 3 , Binglin Sui 1 , Hui Xu 3 , Zhaomin Zheng 3 , Xiaoli Hou 3 , Yonggang Wang 4 , Chenggang Qi 4
Affiliation
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pH- and temperature-sensitive nanogels (NGs) were prepared from sodium alginate (SA) and N-isopropylacrylamide (NIPAM), as the sensitivity at pH 5.5 and 31 °C. SA was pH-modified with glutamic acid (Glu) and ethylenediamine (EDA). The products Glu-SA (Glu-modified SA) and EGSA (EDA- and Glu-modified SA) were characterized by ninhydrin color reaction, infrared spectroscopy, and zeta potential, and the best reactant ratio was selected. Moreover, temperature-sensitive, pH-sensitive EGSA-NGs possessing a semi-interpenetrating network structure were prepared by radical polymerization using N-isopropylacrylamide. The morphology of EGSA-NGs was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM). The cytotoxicity test shows the low cytotoxicity and high biocompatibility of the NGs. The newly prepared NGs were also subjected to pH-sensitive temperature-sensitive in vitro drug-loading and drug-release experiments. The pH-sensitive and temperature-sensitive experiments showed that the particle size of EGSA-NGs was reduced at pH 5.5 and above 31 °C. The drug-loading and drug-release experiments also confirmed this finding, indicating that the newly synthesized NGs could release the drug according to the environmental changes. Therefore, the material has potential application value in solid tumor targeted therapy.
中文翻译：
改性海藻酸钠和NIPAM的温度和pH双响应纳米凝胶用于黄连素的负载和释放
pH和温度敏感的纳米凝胶（NGs）由藻酸钠（SA）和N-异丙基丙烯酰胺（NIPAM）制备，作为对pH 5.5和31°C的敏感度。SA用谷氨酸（Glu）和乙二胺（EDA）进行pH修饰。通过茚三酮显色反应，红外光谱和ζ电势对产物Glu-SA（Glu修饰的SA）和EGSA（EDA和Glu修饰的SA）进行了表征，并选择了最佳的反应物比率。此外，通过使用N进行自由基聚合，制备了具有半互穿网络结构的对温度敏感，对pH敏感的EGSA-NG。-异丙基丙烯酰胺。EGSA-NGs的形态由透射电子显微镜（TEM），扫描电子显微镜（SEM）和原子力显微镜（AFM）表征。细胞毒性测试显示了NG的低细胞毒性和高生物相容性。对新制备的NGs还进行了pH敏感，温度敏感，体外载药和药物释放实验。pH敏感和温度敏感的实验表明，EGSA-NGs的粒径在pH 5.5和高于31°C时减小。载药和释放药物的实验也证实了这一发现，表明新合成的NGs可以根据环境变化释放药物。因此，该材料在实体瘤靶向治疗中具有潜在的应用价值。
更新日期：2021-01-19
中文翻译：
改性海藻酸钠和NIPAM的温度和pH双响应纳米凝胶用于黄连素的负载和释放
pH和温度敏感的纳米凝胶（NGs）由藻酸钠（SA）和N-异丙基丙烯酰胺（NIPAM）制备，作为对pH 5.5和31°C的敏感度。SA用谷氨酸（Glu）和乙二胺（EDA）进行pH修饰。通过茚三酮显色反应，红外光谱和ζ电势对产物Glu-SA（Glu修饰的SA）和EGSA（EDA和Glu修饰的SA）进行了表征，并选择了最佳的反应物比率。此外，通过使用N进行自由基聚合，制备了具有半互穿网络结构的对温度敏感，对pH敏感的EGSA-NG。-异丙基丙烯酰胺。EGSA-NGs的形态由透射电子显微镜（TEM），扫描电子显微镜（SEM）和原子力显微镜（AFM）表征。细胞毒性测试显示了NG的低细胞毒性和高生物相容性。对新制备的NGs还进行了pH敏感，温度敏感，体外载药和药物释放实验。pH敏感和温度敏感的实验表明，EGSA-NGs的粒径在pH 5.5和高于31°C时减小。载药和释放药物的实验也证实了这一发现，表明新合成的NGs可以根据环境变化释放药物。因此，该材料在实体瘤靶向治疗中具有潜在的应用价值。
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