Polycyclic aromatic hydrocarbons, distributing extensively in the soil, would potentially threaten the soil organisms (Eisenia fetida) by triggering oxidative stress. As a ubiquitous antioxidant enzyme, catalase can protect organisms from oxidative damage. To reveal the potential impact of polycyclic aromatic hydrocarbon pyrene (Pyr) on catalase (CAT) and the possible protective effect of Ascorbic acid (vitamin C), multi-spectral and molecular docking techniques were used to investigate the influence of structure and function of catalase by pyrene. Fluorescence and circular dichroism analysis showed that pyrene would induce the microenvironmental changes of CAT amino acid residues and increase the α-helix in the secondary structure. Molecular simulation results indicated that the main binding force of pyrene around the active center of CAT is hydrogen bonding force. Furthermore, pyrene inhibited catalase activity to 69.9% compared with the blank group, but the degree of inhibition was significantly weakened after vitamin C added into the research group. Cell level experiments showed that pyrene can increase the level of ROS in the body cavity cell of earthworms, and put the cells under the threat of potential oxidative damage. Antioxidants-vitamin C has a protective effect on catalase and maintains the stability of intracellular ROS levels to a certain extent.

在土壤中广泛分布的多环芳烃可能会威胁土壤生物（Eisenia fetida）触发氧化应激。过氧化氢酶作为一种普遍存在的抗氧化酶，可以保护生物体免受氧化损伤。为了揭示多环芳烃pyr（Pyr）对过氧化氢酶（CAT）的潜在影响以及抗坏血酸（维生素C）的可能的保护作用，使用多光谱和分子对接技术研究过氧化氢酶的结构和功能的影响。 pyr。荧光和圆二色性分析表明，would会诱导CAT氨基酸残基的微环境变化，并增加二级结构中的α-螺旋。分子模拟结果表明，around在CAT活性中心周围的主要结合力是氢键力。此外，与空白组相比，pyr将过氧化氢酶活性抑制至69.9％，但在研究组中加入维生素C后，抑制程度明显减弱。细胞水平的实验表明，pyr可以提高worm体腔细胞中ROS的水平，并使这些细胞受到潜在的氧化损伤的威胁。抗氧化剂维生素C对过氧化氢酶具有保护作用，并在一定程度上保持细胞内ROS水平的稳定性。