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Lasting Tracking and Rapid Discrimination of Live Gram-Positive Bacteria by Peptidoglycan-Targeting Carbon Quantum Dots
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2021-01-04 , DOI: 10.1021/acsami.0c19651 Chaoren Yan 1 , Chaoli Wang 2 , Tongtong Hou 1 , Ping Guan 1 , Youbei Qiao 2 , Liulong Guo 1 , Yonggang Teng 3 , Xiaoling Hu 1 , Hong Wu 2
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2021-01-04 , DOI: 10.1021/acsami.0c19651 Chaoren Yan 1 , Chaoli Wang 2 , Tongtong Hou 1 , Ping Guan 1 , Youbei Qiao 2 , Liulong Guo 1 , Yonggang Teng 3 , Xiaoling Hu 1 , Hong Wu 2
Affiliation
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Selective discrimination and lasting tracking of live bacteria are primary steps for microbiology research and treatment of bacterial infection. However, conventional detection methods, such as the gold standard of Gram staining, are being challenged under actual test conditions. Herein, we provided a novel method, namely, three excitation peaks and single-color emission carbon quantum dots (T-SCQDs) for the rapid (5 min) peptidoglycan-targeting discrimination of Gram-positive bacteria and lasting tracking (24 h) through one-step staining. Bacterial viability testing indicates that T-SCQDs can achieve nondestructive identification of Gram-positive bacteria within 50–500 μg mL–1. Interestingly, the fluorescence imaging system suggests that T-SCQDs can also selectively distinguish the type of colonies based on fluorescence intensity. Furthermore, T-SCQDs were successfully used to visually distinguish Gram-positive bacteria from the microbial environment of A549 cells by confocal fluorescence microscopy. These properties endow T-SCQDs with excellent functions for the diagnosis of infection and other biological applications.
中文翻译：
肽聚糖靶向碳量子点对革兰氏阳性细菌的持久跟踪和快速区分
选择性区分和持久追踪活细菌是微生物学研究和细菌感染治疗的主要步骤。但是，常规检测方法，例如革兰氏染色的金标准，在实际测试条件下受到了挑战。在此，我们提供了一种新颖的方法，即三个激发峰和单色发射碳量子点（T-SCQD），用于革兰氏阳性细菌的快速（5分钟）肽聚糖靶向识别和持续跟踪（24小时）。一步染色。细菌生存力测试表明，T-SCQD可在50–500μgmL –1范围内实现革兰氏阳性细菌的无损鉴定。有趣的是，荧光成像系统表明，T-SCQD还可以根据荧光强度选择性区分菌落类型。此外，通过共聚焦荧光显微镜，已成功地将T-SCQD用于从A549细胞的微生物环境中目视识别革兰氏阳性细菌。这些特性使T-SCQD具有出色的诊断感染和其他生物学应用的功能。
更新日期：2021-01-13
中文翻译：
肽聚糖靶向碳量子点对革兰氏阳性细菌的持久跟踪和快速区分
选择性区分和持久追踪活细菌是微生物学研究和细菌感染治疗的主要步骤。但是，常规检测方法，例如革兰氏染色的金标准，在实际测试条件下受到了挑战。在此，我们提供了一种新颖的方法，即三个激发峰和单色发射碳量子点（T-SCQD），用于革兰氏阳性细菌的快速（5分钟）肽聚糖靶向识别和持续跟踪（24小时）。一步染色。细菌生存力测试表明，T-SCQD可在50–500μgmL –1范围内实现革兰氏阳性细菌的无损鉴定。有趣的是，荧光成像系统表明，T-SCQD还可以根据荧光强度选择性区分菌落类型。此外，通过共聚焦荧光显微镜，已成功地将T-SCQD用于从A549细胞的微生物环境中目视识别革兰氏阳性细菌。这些特性使T-SCQD具有出色的诊断感染和其他生物学应用的功能。
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