Diisononyl phthalate (DINP) as one of the most commonly used phthalates, has been found in various environmental samples and is considered to have potential risks to ecosystem. Till now, DINP has no clear effect consensus on insects from development to behavior and even mechanisms. Here, Drosophila melanogaster was selected as model organisms and the toxic effects of DINP (0.1%, 0.2%, 0.5% and 1.0%) (v/v) on its metamorphosis, crawling behavior, intestinal cells and cellular redox balance were investigated. During metamorphosis process, lower hatching rate, longer development time, lighter body weight and malformation were observed at high concentration groups. The crawling ability of larvae was severely inhibited by DINP and the movement distance was drastically reduced. DINP could cause severe damage to the larval intestinal cells in the dose-dependent and time-dependent manners. DINP was found to induce redox imbalance with activities of two important antioxidant enzymes (catalase (CAT) and superoxide dismutase (SOD)) increasing, and reactive oxygen species (ROS) level fluctuation in larvae. Our findings provide theoretical basis and data support for scientific management of DINP to reduce ecological risk.

邻苯二甲酸二异壬酯（DINP）是最常用的邻苯二甲酸盐之一，已在各种环境样品中发现，并被认为对生态系统具有潜在风险。到目前为止，DINP对昆虫从发育到行为乃至机制的效果尚无明确共识。在这里，果蝇被选为模型生物，DINP的毒性作用（0.1％，0.2％，0.5％和1.0％）（v/ v）对其变态，爬行行为，肠细胞和细胞氧化还原平衡进行了研究。在变态过程中，高浓度组观察到较低的孵化率，较长的发育时间，较轻的体重和畸形。幼虫的爬行能力受到DINP的严重抑制，运动距离大大减小。DINP可能以剂量依赖性和时间依赖性方式严重破坏幼虫肠道细胞。发现DINP会导致氧化还原失衡，而这两种重要的抗氧化剂酶（过氧化氢酶（CAT）和超氧化物歧化酶（SOD））的活性增加，而幼虫中的活性氧（ROS）含量波动。我们的发现为DINP的科学管理降低生态风险提供了理论基础和数据支持。