The demands of energy sustainability drive efforts to bio-chemical conversion of biomass into biofuels through pretreatment, enzymatic hydrolysis, and microbial fermentation. Pretreatment leads to significant structural changes of the complex lignin polymer that affect yield and productivity of the enzymatic conversion of lignocellulosic biomass. Structural changes of lignin after pretreatment include functional groups, inter unit linkages and compositions. These changes influence non-productive adsorption of enzyme on lignin through hydrophobic interaction and electrostatic interaction as well as hydrogen bonding. This paper reviews the relationships between structural changes of lignin and enzymatic hydrolysis of pretreated lignocellulosic biomass. The formation of pseudo-lignin during dilute acid pretreatment is revealed, and their negative effect on enzymatic hydrolysis is discussed.

中文翻译：

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预处理木质纤维素生物质的酶促转化：木质素结构变化影响的研究进展


能源可持续性的需求推动了通过预处理、酶水解和微生物发酵将生物质生物化学转化为生物燃料的努力。预处理导致复合木质素聚合物的显着结构变化，从而影响木质纤维素生物质酶促转化的产量和生产率。预处理后木质素的结构变化包括官能团、单元间键和组成。这些变化通过疏水相互作用和静电相互作用以及氢键影响酶在木质素上的非生产性吸附。本文综述了木质素结构变化与预处理木质纤维素生物质酶解之间的关系。揭示了稀酸预处理过程中假木质素的形成，并讨论了它们对酶水解的负面影响。