人乳中主要结构化甘油三酯对体外肝细胞脂质代谢的影响,Journal of Agricultural and Food Chemistry

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人乳中主要结构化甘油三酯对体外肝细胞脂质代谢的影响
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-12-28 , DOI: 10.1021/acs.jafc.0c06976
Yanping Wu ₁ , Niu Zhang ₁ , Ze-Yuan Deng ₁ , Hong Zhang ₂ , Jing Li ₁

Affiliation

结构化三酰甘油 [1-油酰-2-棕榈酰-3-亚油酰甘油 (OPL)、3-二亚油酰-2-棕榈酰甘油 (LPL) 和 1,3-二油酰-2-棕榈酰甘油 (OPO)] 在人乳中的作用脂质代谢尚不清楚。因此，本研究调查了不同结构的三酰基甘油及其混合物 (M)（M1、M2 和 M3 中的 OPL/LPL/OPO 为 1.5:0.5:1、1.2:1.2:1 和 0.5:0.2:1，分别）对参与 LO2 细胞脂质代谢的一些关键蛋白质的脂质和表达水平。结果表明，与 OPL、LPL 和 OPO 相比，暴露于 2,3-dioleoyl-1-palmitoylglycerol (POO) 的 LO2 细胞的脂质积累更多（p < 0.05），与 OPL 组相比，OPL 组的脂质积累更多到 LPL 和 OPO 组 ( p< 0.05)。此外，与 M1 和 M2 组相比，M3 组的脂质积累更多。与LPL、OPO和OPL组相比，POO组中二酰基甘油酰基转移酶的表达水平最高，M3组高于M1和M2组。与 OPO 和 LPL 组相比，OPL 组乙酰辅酶 A 羧化酶 1 和长链酰基辅酶 A 合成酶 1 的表达水平最高。与OPO和LPL相比，OPL似乎更可能增加LO2细胞中三酰甘油和胆固醇的含量；因此，这是否有利于婴儿的生长发育还需要进一步验证。

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Effects of the Major Structured Triacylglycerols in Human Milk on Lipid Metabolism of Hepatocyte Cells in Vitro

The effect of structured triacylglycerols [1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL), 3-dilinoleoyl-2-palmitoylglycerol (LPL), and 1,3-dioleoyl-2-palmitoylglycerol (OPO)] in human milk on the lipid metabolism was unclear. Hence, this study investigated the effects of different structured triacylglycerols and their mixtures (M) (OPL/LPL/OPO in M1, M2, and M3 were 1.5:0.5:1, 1.2:1.2:1, and 0.5:0.2:1, respectively) on lipid and expression levels of some critical proteins involved in lipid metabolism in LO2 cells. Results showed that there was more lipid accumulation in the LO2 cells exposed to 2,3-dioleoyl-1-palmitoylglycerol (POO) than OPL, LPL, and OPO (p < 0.05), and more lipid accumulation was observed in the OPL group compared to LPL and OPO groups (p < 0.05). Moreover, there was more lipid accumulation in the M3 group compared to M1 and M2 groups. The expression level of diacylglycerol acyltransferase was highest in the POO group compared to LPL, OPO, and OPL groups and was higher in the M3 group than M1 and M2 groups. The expression levels of acetyl-CoA carboxylase 1 and long-chain acyl-CoA synthetase 1 were highest in the OPL group compared to OPO and LPL groups. In comparison to OPO and LPL, OPL seemed to be more likely to increase the content of triacylglycerols and cholesterol in LO2 cells; therefore, whether this was beneficial to the growth and development of infants needs further verification.

更新日期：2020-12-28

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