Pseudomonas sp. HXF1, a strain capable of heterotrophic nitrification, aerobic denitrification (HNAD), and biomineralization was identified and employed for the simultaneous removal of ammonia nitrogen (NH₄⁺-N) and fluoride (F⁻). It removed 99.2% of NH₄⁺-N without accumulation of nitrous nitrogen (NO₂⁻-N) and nitrate nitrogen (NO₃⁻-N), while removed 87.3% of F⁻. Response surface methodology (RSM) was used to study the best removal conditions for NH₄⁺-N and F⁻. The results of nitrogen balance experiments with NH₄Cl, NaNO₂, and NaNO₃ as single nitrogen sources and amplification experiments of denitrification genes proved that the bacterial strains may remove NH₄⁺-N through HNAD. The experimental results of Scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffractometer (XRD) indicated that the way of F⁻ removal may be adsorption and co-precipitation. The results demonstrated that the strain HXF1 has great potential in the biological denitrification and F⁻ removal of groundwater.

假单胞菌属。HXF1，能够异养硝化的菌株，好氧反硝化（HNAD），和生物矿化被确定，并用于同时除去氨态氮的（NH ₄ ⁺ N）和氟化物（F ^- ）。它除去99.2％的NH ₄ ⁺ -N而不亚硝酸氮的积累（NO ₂ ^- N）和硝酸态氮（NO ₃ ^- -N），而除去的F 87.3％^- 。响应面分析法（RSM）被用来研究对NH最好除去条件₄ ⁺ -N和F ^- 。用NH ₄ Cl，NaNO进行氮平衡实验的结果₂，以NaNO ₃为单一氮源，通过反硝化基因扩增实验，证明该菌株可通过HNAD去除NH ₄ ⁺ -N。扫描电子显微镜的实验结果（SEM），X射线光电子能谱（XPS），和X射线衍射（XRD）表明，F的方式^-去除可以是吸附和共沉淀。结果表明，该菌株HXF1在生物脱氮和F潜力巨大^-去除地下水。